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Identification oftryptophan oxidationproducts in bovineα‐crystallin E FinleyJames DillonRosalie K. Crouch1998 год

Identification of tryptophan oxidation products in bovine α‐crystallin
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Аннотация: Abstract Oxidation is known to affect the structure, activity, and rate of degradation of proteins, and is believed to contribute to a variety of pathological conditions. Metal‐catalyzed oxidation (MCO) is a primary oxidizing system in many cell types. In this study, the oxidative effects of a MCO system (the Fenton reaction) on the structure of the tryptophan residues of α‐crystallin were determined. Tandem mass spectrometry (MS/MS) was utilized to identify specific tryptophan and methionine oxidation products in the bovine α‐crystallin sequence. After oxidative exposure, α‐crystallin was digested with trypsin, and the resulting peptides were fractionated by reverse‐phase HPLC. Structural analysis by mass spectrometry revealed that tryptophan 9 of αA‐ and tryptophan 60 of αB‐crystallin were each converted into hydroxytryptophans (HTRP), N‐formylkynurenine (NFK), and kynurenine (KYN). However, only HTRP and KYN formation were detected at residue 9 of αB‐crystallin. Oxidation of methionine 1 of αA‐ and methionine 1 and 68 of αB‐crystallin was also detected. The products NFK and KYN are of particular importance in the lens, as they themselves are photosensitizers that can generate reactive oxygen species (ROS) upon UV light absorption. The unambiguous identification of HTRP, NFK, and KYN in intact α‐crystallin represents the first structural proof of the formation of these products in an intact protein, and provides a basis for detailed structural analysis of oxidized proteins generated in numerous pathological conditions.
Год издания: 1998
Авторы: E Finley, James Dillon, Rosalie K. Crouch, Kevin L. Schey
Издательство: Wiley
Источник: Protein Science
Ключевые слова: Connexins and lens biology, Biochemical effects in animals, Redox biology and oxidative stress
Другие ссылки: Protein Science (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
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