Аннотация:Contents Most follicles undergo atresia during the developmental process. Follicular atresia is predominantly regulated by apoptosis of granulosa cells, but the mechanism underlying apoptosis via the mitochondria‐dependent apoptotic pathway is unclear. We aimed to investigate whether the mitochondria‐associated genes peroxisome proliferator‐activated receptor‐gamma, coactivator1‐alpha ( PPARGC 1A ), nuclear respiratory factor‐1 ( NRF ‐1 ), B‐cell CLL /lymphoma 2 ( BCL ‐2 ) and BCL 2‐associated X protein ( BAX ) played a role in follicular atresia through this pathway. The four mitochondria‐associated proteins ( PGC ‐1 α , which are encoded by the PPARGC 1A gene, NRF ‐1, BCL ‐2 and BAX ) mainly expressed in granulosa cells. The mRNA and protein levels of PPARGC 1A / PGC ‐1 α and NRF ‐1 in granulosa cells increased with the follicular development. These results showed that these genes may play a role in the regulation of the follicular development. In addition, compared with healthy follicles, the granulosa cell in atretic follicles had a reduced expression of NRF ‐1, increased BAX expression and increased ratio of BAX to BCL ‐2 expression. These results suggested that changes of the mitochondria‐associated gene expression patterns in granulosa cells may lead to follicular atresia during goat follicle development.
