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Optimization offactors affectingAgrobacterium-mediated transformation ofMicro-Tom tomatoes Ming GuoY.L. ZhangZhao Meng2012 год

Optimization of factors affecting Agrobacterium-mediated transformation of Micro-Tom tomatoes
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Страница публикацииПубликация в OpenAlex
Аннотация: Micro-Tom is the smallest known variety of tomatoes.An orthogonal experimental design L 16 (4 5 ) was used to optimize Agrobacterium-mediated transformation of cotyledon explants of Lycopersicon esculentum cv.Micro-Tom.Four parameters were investigated to determine their effect on transformation frequency: the concentration of bacterial suspension, time of dip in bacterial suspension, co-cultivation time, and concentration of carbenicillin.We also examined the effect of these parameters on contamination rate, necrosis rate, mortality, cutsurface browning rate, and undamaged explant rate.Both the bacterial and carbenicillin concentrations had a significant influence on the rate of infected explants.The time of co-cultivation also had a significant influence on the transformation parameters.The optimal transformation protocol consisted of an Agrobacterium suspension of 0.5 × 10 8 cells/mL (OD 600 = 0.5) and an infection time of 5 min, one day of co-cultivation and 500 mg/L carbenicillin.Under these conditions, the transformation efficiency of the shoots reached 5.1%; the mean transfor-©FUNPEC-RP www.funpecrp.com.
Год издания: 2012
Авторы: Ming Guo, Y.L. Zhang, Zhao Meng, Jing Jiang
Издательство: Research Foundation of Ribeirão Preto
Источник: Genetics and Molecular Research
Ключевые слова: Plant tissue culture and regeneration, Transgenic Plants and Applications, Plant Virus Research Studies
Другие ссылки: Genetics and Molecular Research (HTML)
PubMed (HTML)