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Toxicity ofribosome-inactivatingproteins-containingimmunotoxins to ahuman bladdercarcinoma cell line Maria Giulia BattelliLetizia PolitoAndrea Bolognesi1996 год

Toxicity of ribosome-inactivating proteins-containing immunotoxins to a human bladder carcinoma cell line
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Аннотация: Immunotoxins were prepared by linking the type I ribosome-inactivating proteins (RIP) momordin I, pokeweed antiviral protein from seeds (PAP-S) and saporin-S6 to the 48-127 monoclonal antibody (MAb) recognising a glycoprotein (gp54) expressed on all human bladder tumours tested and on human bladder carcinoma cell lines, in particular on the T24 cell line. T24 cells required a 2 hr contact with immunotoxins to ensure binding and endocytosis. A time course of exposure, followed by further incubation without the immunotoxins, showed that maximum inhibition of protein synthesis by T24 cells was reached after 2 hr of contact followed by 3 days without the immunotoxins. Under optimal conditions, 48-127/RIP immunotoxins at nanomolar concentrations inhibited by 50% protein synthesis of target T24 cells. No toxicity was observed if (i) target cells were treated with non-conjugated RIP, (ii) target cells were treated with momordin I- or PAP-S-containing immunotoxins made with an irrelevant antibody and (iii) a non-target cell line was treated with the same 2 RIP conjugated to 48-127 antibody. The in vitro selective toxicity of these immunotoxins encourages further studies in view of a possible use in clinical trials for the local therapy of human bladder carcinomas. © 1996 Wiley-Liss, Inc.
Год издания: 1996
Авторы: Maria Giulia Battelli, Letizia Polito, Andrea Bolognesi, Louis Lafleur, Yves Fradet, Fiorenzo Stirpe
Издательство: Wiley
Источник: International Journal of Cancer
Ключевые слова: Toxin Mechanisms and Immunotoxins, Transgenic Plants and Applications, Plant Virus Research Studies
Другие ссылки: International Journal of Cancer (PDF)
International Journal of Cancer (HTML)
PubMed (HTML)