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Cyclin E-induced Sphase withoutactivation of thepRb/E2F pathway. Jiří LukášThomas HerzingerKlaus Hansen1997 год

Cyclin E-induced S phase without activation of the pRb/E2F pathway.
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Аннотация: In cells of higher eukaryotes, cyclin D-dependent kinases Cdk4 and Cdk6 and, possibly, cyclin E-dependent Cdk2 positively regulate the G1- to S-phase transition, by phosphorylating the retinoblastoma protein (pRb), thereby releasing E2F transcription factors that control S-phase genes. Here we performed microinjection and transfection experiments using rat R12 fibroblasts, their derivatives conditionally overexpressing cyclins D1 or E, and human U-2-OS cells, to explore the action of G1 cyclins and the relationship of E2F and cyclin E in S-phase induction. We demonstrate that ectopic expression of cyclin E, but not cyclin D1, can override G1 arrest imposed by either the p16INK4a Cdk inhibitor specific for Cdk4 and Cdk6 or a novel phosphorylation-deficient mutant pRb. Several complementary approaches to assess E2F activation, including quantitative reporter assays in live cells, showed that the cyclin E-induced S phase and completion of the cell division cycle can occur in the absence of E2F-mediated transactivation. Together with the ability of cyclin E to overcome a G1 block induced by expression of dominant-negative mutant DP-1, a heterodimeric partner of E2Fs, these results provide evidence for a cyclin E-controlled S phase-promoting event in somatic cells downstream of or parallel to phosphorylation of pRb and independent of E2F activation. They furthermore indicate that a lack of E2F-mediated transactivation can be compensated by hyperactivation of this cyclin E-controlled event.
Год издания: 1997
Авторы: Jiří Lukáš, Thomas Herzinger, Klaus Hansen, Maria Cristina Moroni, Dalia Resnitzky, Kristian Helin, Steven I. Reed, Jiří Bártek
Издательство: Cold Spring Harbor Laboratory Press
Источник: Genes & Development
Ключевые слова: Cancer-related Molecular Pathways, Epigenetics and DNA Methylation, Genomics and Chromatin Dynamics
Другие ссылки: Genes & Development (PDF)
Genes & Development (HTML)
PubMed (HTML)