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Activated regulatoryT-cells attenuatemyocardialischaemia/reperfusioninjury through aCD39-dependentmechanism Ni XiaJiao JiaoTingting Tang2015 год

Activated regulatory T-cells attenuate myocardial ischaemia/reperfusion injury through a CD39-dependent mechanism
статья из журнала

Страница публикацииПубликация в OpenAlex
Аннотация: Regulatory T-cells (Tregs) are generally regarded as key immunomodulators that maintain immune tolerance and counteract tissue damage in a variety of immune-mediated disorders. However, its role in myocardial ischaemia/reperfusion injury (MIRI) remains unknown. The purpose of the present study was to determine whether Tregs exert a beneficial effect on mouse MIRI. We examined the role of Tregs in murine MIRI by depletion using 'depletion of regulatory T-cell' (DEREG) mice and adoptive transfer using Forkhead box P3 (Foxp3)-GFP knockin mice and the mechanisms of cardio protection were further studied in vivo and in vitro. Tregs rapidly accumulated in murine hearts following MIRI. Selective depletion of Tregs in the DEREG mouse model resulted in aggravated MIRI. In contrast, the adoptive transfer of in vitro-activated Tregs suppressed MIRI, whereas freshly isolated Tregs had no effect. Mechanistically, activated Treg-mediated protection against MIRI was not abrogated by interleukin (IL)-10 or transforming growth factor (TGF)-β1 inhibition but was impaired by the genetic deletion of cluster of differentiation 39 (CD39). Moreover, adoptive transfer of in vitro-activated Tregs attenuated cardiomyocyte apoptosis, activated a pro-survival pathway involving Akt and extracellular-signal-regulated kinase (ERK) and inhibited neutrophil infiltration, which was compromised by CD39 deficiency. Finally, the peripheral blood mononuclear cells of acute myocardial infarction (AMI) patients after primary percutaneous coronary intervention (PCI) revealed a decrease in CD4+CD25+CD127low Tregs and a relative increase in CD39+ cells within the Treg population. In conclusion, our data validated a protective role for Tregs in MIRI. Moreover, in vitro-activated Tregs ameliorated MIRI via a CD39-dependent mechanism, representing a putative therapeutic strategy.
Год издания: 2015
Авторы: Ni Xia, Jiao Jiao, Tingting Tang, Bing-Jie Lv, Yu-Zhi Lu, Ke-Jing Wang, Zheng-Feng Zhu, Xiao-Bo Mao, Shao-Fang Nie, Qing K. Wang, Xin Tu, Hong Xiao, Yu-Hua Liao, Guo‐Ping Shi, Xiang Cheng
Издательство: Portland Press
Источник: Clinical Science
Ключевые слова: Neuroinflammation and Neurodegeneration Mechanisms, Immune Cell Function and Interaction, Adenosine and Purinergic Signaling
Другие ссылки: Clinical Science (HTML)
PubMed (HTML)