Аннотация:A significant proportion of P‐glycoprotein (P‐gp) and caveolin was co‐localized in caveolae isolated from resistant (CH R C5) cells overexpressing P‐gp and from drug‐sensitive Chinese hamster ovary cells (AuxB1). The proportion of P‐gp and caveolin associated with caveolar microdomains was higher in CH R C5 cells grown in the presence of P‐gp substrates (cyclosporin A or colchicine) than in untreated CH R C5 cells. Co‐immunoprecipitation of P‐gp and caveolin from CH R C5 lysates suggests that there is a physical interaction between them. Furthermore, co‐localization of P‐gp and caveolin was found in caveolae from brain capillaries, indicating that this association also takes place in vivo.
