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High Resolution MALDIImaging MassSpectrometry ofRetinal Tissue Lipids David M. AndersonZsolt AblonczyYiannis Koutalos2014 год

High Resolution MALDI Imaging Mass Spectrometry of Retinal Tissue Lipids
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Аннотация: Matrix assisted laser desorption ionization imaging mass spectrometry (MALDI IMS) has the ability to provide an enormous amount of information on the abundances and spatial distributions of molecules within biological tissues. The rapid progress in the development of this technology significantly improves our ability to analyze smaller and smaller areas and features within tissues. The mammalian eye has evolved over millions of years to become an essential asset for survival, providing important sensory input of an organism's surroundings. The highly complex sensory retina of the eye is comprised of numerous cell types organized into specific layers with varying dimensions, the thinnest of which is the 10 μm retinal pigment epithelium (RPE). This single cell layer and the photoreceptor layer contain the complex biochemical machinery required to convert photons of light into electrical signals that are transported to the brain by axons of retinal ganglion cells. Diseases of the retina, including age-related macular degeneration (AMD), retinitis pigmentosa, and diabetic retinopathy, occur when the functions of these cells are interrupted by molecular processes that are not fully understood. In this report, we demonstrate the use of high spatial resolution MALDI IMS and FT-ICR tandem mass spectrometry in the Abca4(-/-) knockout mouse model of Stargardt disease, a juvenile onset form of macular degeneration. The spatial distributions and identity of lipid and retinoid metabolites are shown to be unique to specific retinal cell layers.
Год издания: 2014
Авторы: David M. Anderson, Zsolt Ablonczy, Yiannis Koutalos, Jeffrey M. Spraggins, Rosalie K. Crouch, Richard M. Caprioli, Kevin L. Schey
Издательство: American Chemical Society
Источник: Journal of the American Society for Mass Spectrometry
Ключевые слова: Metabolomics and Mass Spectrometry Studies, Mass Spectrometry Techniques and Applications, Malaria Research and Control
Другие ссылки: Journal of the American Society for Mass Spectrometry (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
PubMed (HTML)