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Yeast Lrg1p acts as aspecialized RhoGAPregulating1,3‐β‐glucansynthesis Daisuke WatanabeMitsuhiro AbeYoshikazu Ohya2001 год

Yeast Lrg1p acts as a specialized RhoGAP regulating 1,3‐β‐glucan synthesis
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Аннотация: Abstract Selection of an extragenic suppressor of fks1‐1154 Δfks2 , mutations in the catalytic subunits of yeast 1,3‐β‐glucan synthase (GS) conferring temperature‐sensitivity, led to the LRG1 gene, which was originally identified as a L IM‐ R ho G AP homologous gene. Mutations in the LRG1 gene restore impaired 1,3‐β‐glucan synthesis in the fks1‐1154 Δfks2 mutant as well as that in rho1‐2 , a temperature‐sensitive mutant of Rho‐type GTPase that functions as a regulatory subunit of GS. Two‐hybrid analyses of Lrg1p, which contains a sequence conserved among Rho GTPase‐activating proteins (GAPs), revealed its specific interactions with the active form of Rho1p. Among eight potential yeast RhoGAPs, Lrg1p is the only member that negatively regulates GS activity: mutations in the rest of GAPs, including bem2 , Δbem3 , Δsac7 , Δbag7 , Δrga1 , Δrga2 and Δrgd1 , do not suppress impairment of 1,3‐β‐glucan synthesis. Analyses of Mpk1p phosphorylation revealed the inability of Lrg1p to regulate the Pkc1p–MAP kinase cascade, a distinct Rho1p‐regulating signalling pathway known to be affected by the GAPs, Bem2p and Sac7p. Thus, different groups of Rho1p GAPs control the activity of different Rho1p‐effector proteins. Copyright © 2001 John Wiley & Sons, Ltd.
Год издания: 2001
Авторы: Daisuke Watanabe, Mitsuhiro Abe, Yoshikazu Ohya
Издательство: Wiley
Источник: Yeast
Ключевые слова: Fungal and yeast genetics research, Endoplasmic Reticulum Stress and Disease, Polysaccharides and Plant Cell Walls
Другие ссылки: Yeast (HTML)
PubMed (HTML)