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Multiplecis-actingregulatory regionsare required forrestrictedspatio-temporalHoxa5gene expression Christian LarochelleMichel J. TremblayDaniel Bernier1999 год

Multiplecis-acting regulatory regions are required for restricted spatio-temporalHoxa5 gene expression
статья из журнала

Страница публикацииПубликация в OpenAlex
Аннотация: Genetic analyses have revealed the essential role of the murine Hoxa5 gene for the correct specification of the cervical and upper thoracic region of the skeleton, and for the normal organogenesis and function of the respiratory tract, both structures expressing Hoxa5 during embryogenesis. To understand how the expression domains of the Hoxa5 gene are established during development, we have analyzed the cis-acting control regions mediating Hoxa5 gene expression using a transgenic approach. Four transcripts are derived from the Hoxa5 locus. The shortest and most abundant one displays a specific spatio-temporal profile of expression at earlier stages and in more anterior structures along the embryonic axis than the larger forms. We established that an 11.1 kilobase pair (kb) genomic fragment, extending from position −3.8 kb to +7.3 kb relative to Hoxa5 transcription initiation site, was sufficient to reproduce the temporal expression and substantially reconstitute the spatial pattern of the major Hoxa5 transcript. By deletion analyses, we identified a 2.1 kb fragment located downstream of the Hoxa5 gene that possesses mesodermal enhancer activity. Overall, the findings demonstrate that cis-acting regulatory elements essential for the correct expression of the major Hoxa5 transcript are located both upstream and downstream of the Hoxa5 coding sequences. Dev Dyn 1999;214:127–140. © 1999 Wiley-Liss, Inc.
Год издания: 1999
Авторы: Christian Larochelle, Michel J. Tremblay, Daniel Bernier, Jos�e Aubin, Lucie Jeannotte
Издательство: Wiley
Источник: Developmental Dynamics
Ключевые слова: Developmental Biology and Gene Regulation, Genomics and Chromatin Dynamics, Cancer-related gene regulation
Другие ссылки: Developmental Dynamics (HTML)
PubMed (HTML)