Аннотация:We demonstrate that in Drosophila melanogaster the histone H3.3 replacement variant is encoded by two genes, H3.3A and H3.3B. We have isolated cDNA clones for H3.3A and cDNA and genomic clones for H3.3B. The genes encode exactly the same protein but are widely divergent in their untranslated regions (UTR). Both genes are expressed in embryos and adults; they are expressed in the gonads as well as in somatic tissues of the flies. However, only one of them, H3.3A, shows strong testes expression. The 3′ UTR of the H3.3A gene is relatively short (~250 nucleotides (nt)). H3.3B transcripts can be processed at several polyadenylation sites, the longest with a 3′ UTR of more than 1500 nt. The 3′ processing sites, preferentially used in the gonads and somatic tissues, are different. We have also isolated the Drosophila hydei homologues of the two H3.3 genes. They are quite similar to the D. melanogaster genes in their expression patterns. However, in contrast to their vertebrate counterparts, which are highly conserved in their noncoding regions, the Drosophila genes display only limited sequence similarity in these regions.Key words: H3.3 histone variant, Drosophila, sequence comparison, alternative polyadenylation, testis expression.
Ключевые слова:Chromosomal and Genetic Variations, Genomics and Chromatin Dynamics, Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
