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Calpain 2 is requiredfor the invasion ofglioblastoma cells inthe zebrafish brainmicroenvironment Sangeet LalJane La DuRobert L. Tanguay2011 год

Calpain 2 is required for the invasion of glioblastoma cells in the zebrafish brain microenvironment
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Аннотация: Abstract Glioblastoma is an aggressive primary brain tumor with a 5‐year survival rate of less than 5%. The ability of glioblastoma cells to invade surrounding brain tissue presents the primary challenge for the success of focal therapeutic approaches. We previously reported that the calcium‐activated protease calpain 2 is critical for glioblastoma cell invasion in vitro. Here, we show that expression of calpain 2 is required for the dispersal of glioblastoma cells in a living brain microenvironment. Knockdown of calpain 2 resulted in a 2.9‐fold decrease in the invasion of human glioblastoma cells in zebrafish brain. Control cells diffusely migrated up to 450 μm from the site of injection, whereas knockdown cells remained confined in clusters. The invasion study was repeated in organotypic mouse brain tissues, and calpain 2 knockdown cells demonstrated a 2.3‐fold lower area of dispersal compared with control cells. In zebrafish brain, glioblastoma cells appeared to migrate in part along the blood vessels of the host. Furthermore, angiogenesis was detected in 27% of zebrafish injected with control cells, whereas only 12.5% of fish receiving knockdown cells showed the formation of new vessels, suggesting a role for calpain 2 in tumor cell angiogenesis. Consistent with the progression of glioblastoma in humans, transplanted tumor cells were not observed to metastasize outside the brain of zebrafish. This study demonstrates that calpain 2 expression is required for the dispersal of glioblastoma cells within the dynamic microenvironment of the brain, identifying zebrafish as a valuable orthotopic system for studying glioblastoma cell invasion. © 2011 Wiley Periodicals, Inc.
Год издания: 2011
Авторы: Sangeet Lal, Jane La Du, Robert L. Tanguay, Jeffrey A. Greenwood
Издательство: Wiley
Источник: Journal of Neuroscience Research
Ключевые слова: Calpain Protease Function and Regulation, Zebrafish Biomedical Research Applications, Neuroinflammation and Neurodegeneration Mechanisms
Другие ссылки: Journal of Neuroscience Research (HTML)
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