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The Na+/I− symportermediates activeiodide uptake in theintestine Juan P. NicolaCécile BasquinCarla Portulano2008 год

The Na+/I− symporter mediates active iodide uptake in the intestine
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Аннотация: Absorption of dietary iodide, presumably in the small intestine, is the first step in iodide (I − ) utilization. From the bloodstream, I − is actively taken up via the Na + /I − symporter (NIS) in the thyroid for thyroid hormone biosynthesis and in such other tissues as lactating breast, which supplies I − to the newborn in the milk. The molecular basis for intestinal I − absorption is unknown. We sought to determine whether I − is actively accumulated by enterocytes and, if so, whether this process is mediated by NIS and regulated by I − itself. NIS expression was localized exclusively at the apical surface of rat and mouse enterocytes. In vivo intestine-to-blood transport of pertechnetate, a NIS substrate, was sensitive to the NIS inhibitor perchlorate. Brush border membrane vesicles accumulated I − in a sodium-dependent, perchlorate-sensitive manner with kinetic parameters similar to those of thyroid cells. NIS was expressed in intestinal epithelial cell line 6, and I − uptake in these cells was also kinetically similar to that in thyrocytes. I − downregulated NIS protein expression and its own NIS-mediated transport both in vitro and in vivo. We conclude that NIS is functionally expressed on the apical surface of enterocytes, where it mediates active I − accumulation. Therefore, NIS is a significant and possibly central component of the I − absorption system in the small intestine, a system of key importance for thyroid hormone biosynthesis and thus systemic intermediary metabolism.
Год издания: 2008
Авторы: Juan P. Nicola, Cécile Basquin, Carla Portulano, Andrea Reyna‐Neyra, Monika Paroder, Nancy Carrasco
Издательство: American Physical Society
Источник: AJP Cell Physiology
Ключевые слова: Thyroid Disorders and Treatments, Neonatal Health and Biochemistry, Chemical Analysis and Environmental Impact
Другие ссылки: AJP Cell Physiology (HTML)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
PubMed (HTML)