1. Главная
  2. Ресурсы
  3. Библиотечный поиск
The multi-PDZ domainprotein MUPP1 is acytoplasmic ligandfor themembrane-spanningproteoglycan NG2 Diana S. BarrittMichael T. PearnAndreas H. Zisch2000 год

The multi-PDZ domain protein MUPP1 is a cytoplasmic ligand for the membrane-spanning proteoglycan NG2
статья из журнала

Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: A yeast two-hybrid screen was employed to identify ligands for the cytoplasmic domain of the NG2 chondroitin sulfate proteoglycan. Two overlapping cDNA clones selected in the screen are identical in sequence to a DNA segment coding for the most amino-terminal of the 13 PDZ domains found in the multi-PDZ-protein MUPP1. Antibodies made against recombinant polypeptides representing these two clones (NIP-2 and NIP-7) are reactive with the same 250-kDa molecule recognized by anti-MUPP1 antibodies, confirming the presence of the NIP-2 and NIP-7 sequences in the MUPP1 protein. NIP-2 and NIP-7 GST fusion proteins effectively recognize NG2 in pull-down assays, demonstrating the ability of these polypeptide segments to interact with the intact proteoglycan. The fusion proteins fail to bind NG2 missing the C-terminal half of the cytoplasmic domain, emphasizing the role of the NG2 C-terminus in the interaction with MUPP1. The existence of an NG2/MUPP1 interaction in situ is demonstrated by the ability of NG2 antibodies to co-immunoprecipitate both NG2 and MUPP1 from detergent extracts of cells expressing the two molecules. MUPP1 may serve as a multivalent scaffold that provides a means of linking NG2 with key structural and/or signaling components in the cytoplasm. J. Cell. Biochem. 79:213–224, 2000. © 2000 Wiley-Liss, Inc.
Год издания: 2000
Авторы: Diana S. Barritt, Michael T. Pearn, Andreas H. Zisch, Siu Sylvia Lee, Ronald T. Javier, Elena B. Pasquale, William B. Stallcup
Издательство: Wiley
Источник: Journal of Cellular Biochemistry
Ключевые слова: Wnt/β-catenin signaling in development and cancer, Hippo pathway signaling and YAP/TAZ, Skin and Cellular Biology Research
Другие ссылки: Journal of Cellular Biochemistry (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
PubMed (HTML)