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Variation betweenstrains of thecyanobacteriumMicrocystisaeruginosa isolatedfrom a Portugueseriver Martin L. SakerJutta FastnerElke Dittmann2005 год

Variation between strains of the cyanobacterium Microcystis aeruginosa isolated from a Portuguese river
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Аннотация: The aim of this study was to investigate toxicological differences between strains of the cyanobacterium Microcystis aeruginosa isolated from a potable water supply in the north of Portugal over a 2-month period.Twenty-six strains of M. aeruginosa were isolated, grown in pure culture, and tested using a range of techniques including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), ELISA and a PCR procedure targeting the genes implicated in the production of toxic microcystins. There was considerable variation with respect to the amounts of microcystin produced by each of the strains as measured by ELISA, with values ranging from 0.02 to 0.53% dry weight. The results of the MALDI-TOF MS analysis demonstrated the presence of several chemically distinct forms of microcystin as well as aeruginosins, anabaenopeptins and several other unidentified peptide-like compounds.The growth of individual strains that comprise bloom populations, with unique 'chemotypes' can potentially be an important factor affecting the toxicity of bloom populations. Molecular probes, targeting the genes responsible for microcystin production were shown to be useful for distinguishing between toxic and nontoxic strains and showed good agreement with the results obtained from the other analyses.The results of this study show that the analysis of cyanobacterial bloom populations at the subspecies (strain) level can potentially provide important information regarding the toxin-producing potential of a cyanobacterial bloom and could be used as an 'early warning' for toxic bloom development.
Год издания: 2005
Авторы: Martin L. Saker, Jutta Fastner, Elke Dittmann, Gunna Christiansen, Vı́tor Vasconcelos
Издательство: Oxford University Press
Источник: Journal of Applied Microbiology
Ключевые слова: Aquatic Ecosystems and Phytoplankton Dynamics, Marine and coastal ecosystems, Marine Toxins and Detection Methods
Другие ссылки: Journal of Applied Microbiology (HTML)
PubMed (HTML)