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Two distinct pathwaysfor the invasion ofStreptococcuspyogenes innon-phagocytic cells Gabriella MolinariManfred RohdeCarlos A. Guzmán2000 год

Two distinct pathways for the invasion of Streptococcus pyogenes in non-phagocytic cells
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Аннотация: Adherence to and invasion of epithelial cells represent important pathogenic mechanisms of Streptococcus pyogenes. A fibronectin-binding surface protein of S. pyogenes, SfbI protein, has been implicated in both adherence and invasion processes. Invasion of SfbI-containing strains has been suspected to be responsible for the failure of antibiotics treatment to eradicate S. pyogenes. In this study, we tested the adherence and invasion properties of two well-characterized clinical isolates: A40, which expresses SfbI; and A8, which is SfbI negative and is unable to bind fibronectin. In strain A40, SfbI was the main factor required for attachment and invasion by using fibronectin as a bridging molecule and the α5β1 integrin as cellular receptor. The uptake process was characterized by the generation of large membrane invaginations at the bacteria–cell interface without evidence of actin recruitment or cellular injury. A40 cells were located in phagosomes and, only 24 h after infection, a consistent part of the bacterial population reached the cytoplasm. In contrast, uptake of strain A8 required major rearrangements of cytoskeletal proteins underneath attached bacteria. In A8, a proteinaceous moiety was involved, which does not interact with α5β1 or need any known bridging molecule. Bacterial attachment stimulated elongation and massive recruitment of neighbouring microvilli, which fused to surround streptococcal chains. They led to the generation of large pseudopod-like structures, which engulfed bacteria that were rapidly released and replicated in the cytoplasm. The identification of two completely different uptake pathways reported here provided further evidence regarding the diversity of S. pyogenes isolates and might contribute towards understanding the pathogenesis and persistence of S. pyogenes.
Год издания: 2000
Авторы: Gabriella Molinari, Manfred Rohde, Carlos A. Guzmán, Gursharan S. Chhatwal
Издательство: Wiley
Источник: Cellular Microbiology
Ключевые слова: Streptococcal Infections and Treatments, Antimicrobial Resistance in Staphylococcus, Neonatal and Maternal Infections
Другие ссылки: Cellular Microbiology (HTML)
PubMed (HTML)