Аннотация:Abstract: We have expressed and biochemically characterized the human D2 long (D2 L ) dopamine receptor isoform using the baculovirus/Sf9 cell system. The expressed receptor bound ligands with a pharmacological profile similar to that reported for neuronal and cloned D2 L receptors expressed in mammalian cell lines. Dopamine binding to D2 L receptor was sensitive to guanine nucleotides, indicating receptor coupling to endogenous G proteins. A D2 L receptor‐specific antibody identified two major protein species at ∼44 kDa and at ∼93 kDa in immunoblots, suggesting the presence of D2 L receptor monomers and dimers. Both species were purified by immunoprecipitation from digitonin‐solubilized preparation of cells expressing D2 L receptor prelabeled with 32 P i or [ 3 H]‐palmitate. These results constitute the first direct evidence for D2 L receptor phosphorylation and palmitoylation.
