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Regulation of twodistinct alcoholoxidase promoters inthe methylotrophicyeast Pichiamethanolica Tomoyuki NakagawaAtsushi InagakiTakashi Ito2006 год

Regulation of two distinct alcohol oxidase promoters in the methylotrophic yeast Pichia methanolica
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Аннотация: Abstract In this study, two Pichia methanolica alcohol oxidase (AOD) promoters, P MOD 1 and P MOD 2 , were evaluated in a promoter assay system utilizing the acid phosphatase (AP) gene from Saccharomyces cerevisiae ( ScPHO5 ) as a reporter. Heterologous gene expression driven by the P MOD 1 and P MOD 2 promoters was found to be strong and tightly regulated by carbon source at the transcriptional level. P MOD 1 was induced not only by methanol but also by glycerol. P MOD 2 was induced only by methanol, although it was not repressed on the addition of glycerol to a methanol medium, suggesting that P MOD 2 is regulated in a manner distinct from that of other AOD‐gene promoters. On the other hand, methanol and oxygen level‐influenced gene expression mediated by P MOD 1 and P MOD 2 . P MOD 1 expression was optimal at low methanol concentrations, whereas P MOD 2 was predominantly expressed at high methanol and high oxygen concentrations. Based on these results, both P MOD 2 and P MOD 1 should be useful tools for controlling heterologous gene expression in P. methanolica . In particular, it should be possible to differentially control the production phases of two heterologous proteins, using P MOD 1 and P MOD 2 in the same host cell and in the same flask. Copyright © 2006 John Wiley & Sons, Ltd.
Год издания: 2006
Авторы: Tomoyuki Nakagawa, Atsushi Inagaki, Takashi Ito, Shuki Fujimura, Tatsuro Miyaji, Hiroya Yurimoto, Nobuo Kato, Yasuyoshi Sakai, Noboru Tomizuka
Издательство: Wiley
Источник: Yeast
Ключевые слова: Fungal and yeast genetics research, Fermentation and Sensory Analysis, Biofuel production and bioconversion
Другие ссылки: Yeast (HTML)
PubMed (HTML)