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Zea mays AnnexinsModulate CytosolicFree Ca2+ andGenerate aCa2+-PermeableConductance Anuphon LaohavisitJenny C. MortimerVadim Demidchik2009 год

Zea mays Annexins Modulate Cytosolic Free Ca2+ and Generate a Ca2+-Permeable Conductance
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Аннотация: Abstract Regulation of reactive oxygen species and cytosolic free calcium ([Ca2+]cyt) is central to plant function. Annexins are small proteins capable of Ca2+-dependent membrane binding or membrane insertion. They possess structural motifs that could support both peroxidase activity and calcium transport. Here, a Zea mays annexin preparation caused increases in [Ca2+]cyt when added to protoplasts of Arabidopsis thaliana roots expressing aequorin. The pharmacological profile was consistent with annexin activation (at the extracellular plasma membrane face) of Arabidopsis Ca2+-permeable nonselective cation channels. Secreted annexins could therefore modulate Ca2+ influx. As maize annexins occur in the cytosol and plasma membrane, they were incorporated at the intracellular face of lipid bilayers designed to mimic the plasma membrane. Here, they generated an instantaneously activating Ca2+-permeable conductance at mildly acidic pH that was sensitive to verapamil and Gd3+ and had a Ca2+-to-K+ permeability ratio of 0.36. These results suggest that cytosolic annexins create a Ca2+ influx pathway directly, particularly during stress responses involving acidosis. A maize annexin preparation also demonstrated in vitro peroxidase activity that appeared independent of heme association. In conclusion, this study has demonstrated that plant annexins create Ca2+-permeable transport pathways, regulate [Ca2+]cyt, and may function as peroxidases in vitro.
Год издания: 2009
Авторы: Anuphon Laohavisit, Jenny C. Mortimer, Vadim Demidchik, Katy M. Coxon, Matthew A. Stancombe, Neil Macpherson, Colin Brownlee, Andreas Hofmann, Alex Webb, Henk Miedema, N. H. Battey, Julia M. Davies
Издательство: Oxford University Press
Источник: The Plant Cell
Ключевые слова: S100 Proteins and Annexins, Redox biology and oxidative stress, Connexins and lens biology
Другие ссылки: The Plant Cell (PDF)
The Plant Cell (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
PubMed Central (HTML)
CentAUR (University of Reading) (HTML)
PubMed (HTML)