Аннотация:A vigorous effort has been paid to identify genes for hypertension, however no consistent results have been reported. The candidate gene approach is a corner-stone to identify the susceptible gene associated with hypertension. However, strategy of the current case control study has been limited to examine one or a few genes. In the present study, we used systemic multiple candidate genes approach to identify not only susceptible genes but also susceptible genetic pathway for hypertension. Three hundreds seventeen SNPs with following criteria were evaluated in a Japanese population (hypertensive patients (n=888) and normotensive controls (n=840)) recruited from four regional areas in Japan. (i) SNPs were selected in genes encoding the components of the signal transduction system, i.e. enzymes (66 SNPs), channels (53 SNPs), receptors (48 SNPs), transporters (34 SNPs), G-proteins (32 SNPs), and binding proteins (7 SNPs), which are possibly related to blood pressure regulations. (ii) One SNP in one gene with the highest minor allele frequency in Japanese population was selected. (iii) SNPs were selected from those published in JSNP (http://snp.ims.u-tokyo.ac.jp/index.html). Chi-square analysis was performed for each SNP. Dominant and recessive models, as well as allele frequency were evaluated. P value less than 0.05 was defined as statistically significant. Of 317 SNPs evaluated, 45 SNPs (14.2 %) had a p values less than 0.05. The highest positive rate was observed in genes related to G-proteins (9/32, 28.1%). The validation in a large general population (n=12,000) is now in the process and will be presented.
