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PhosphatidylglucosideExists as a SingleMolecular Specieswith Saturated FattyAcyl Chains inDeveloping AstroglialMembranes Yasuko NagatsukaYasuhiro HoribataYasuhiro Yamazaki2006 год

Phosphatidylglucoside Exists as a Single Molecular Species with Saturated Fatty Acyl Chains in Developing Astroglial Membranes
статья из журнала

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Аннотация: We previously found that phosphatidylglucoside (PtdGlc), a novel glycolipid expressed in HL60 cells, plays a role in forming signaling microdomains involved in cellular differentiation. Because cells contain minute levels of PtdGlc, pure PtdGlc is very difficult to isolate. Thus, its complete structure has never been assessed. To aid in analyzing PtdGlc, we generated a PtdGlc-specific monoclonal antibody, DIM21, by immunizing mice with detergent-insoluble membranes isolated from HL60 cells [Yamazaki, Y., et al. (2006) J. Immunol. Methods 311, 106−116]. DIM21 immunostaining of murine CNS tissues revealed stage- and cell type-specific localization of the DIM21 antigen during development, with especially high levels of expression in radial glia/astroglia. DIM21 immunostained cultured hippocampal astroglia in a punctate fashion. To characterize the structure of PtdGlc, we isolated DIM21 antigen from fetal brains. Using successive column chromatography, we purified two previously unrecognized glycolipids, PGX-1 and PGX-2, from embryonic day 21 rat brains. DIM21 reacted more strongly to PGX-2 than to PGX-1. Structural analyses with 600 MHz 1H NMR, FT-ICR mass spectrometry, and GC revealed that PGX-1 is phosphatidyl β-d-(6-O-acetyl)glucopyranoside and PGX-2 is phosphatidyl β-d-glucopyranoside. The yields of PGX-1 and PGX-2 were approximately 250 ± 150 and 440 ± 270 nmol/g of dried brains, respectively. Surprisingly, both glycolipids were composed exclusively of C18:0 at the C1 position and C20:0 at the C2 position of the glycerol backbone. This saturated fatty acyl chain composition comprising a single molecular species rarely occurs in known mammalian lipids and provides a molecular basis for why PtdGlc resides in raftlike lipid microdomains.
Год издания: 2006
Авторы: Yasuko Nagatsuka, Yasuhiro Horibata, Yasuhiro Yamazaki, Masami O. Kinoshita, Yoko Shinoda, Tsutomu Hashikawa, Hiroyuki Koshino, Takemichi Nakamura, Yoshio Hirabayashi
Издательство: American Chemical Society
Источник: Biochemistry
Ключевые слова: Lipid Membrane Structure and Behavior, Sphingolipid Metabolism and Signaling, Neuroscience and Neuropharmacology Research
Другие ссылки: Biochemistry (HTML)
PubMed (HTML)