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Chromosomalintegration of asynthetic expressioncontrol sequenceachievespoly‐γ‐glutamateproduction in aBacillus subtilisstrain Chuan‐Mei YehJyh‐Perng WangShih‐Ching Lo2010 год

Chromosomal integration of a synthetic expression control sequence achieves poly‐γ‐glutamate production in a Bacillus subtilis strain
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Аннотация: Abstract Poly‐γ‐glutamate (γ‐PGA) has applications in food, medical, cosmetic, animal feed, and wastewater industries. Bacillus subtilis DB430, which possesses the γ‐PGA synthesis ywsC‐ywtAB genes in its chromosome, cannot produce γ‐PGA. An efficient synthetic expression control sequence (SECS) was introduced into the upstream region of the ywtABC genes, and this resulted in γ‐PGA‐producing B. subtilis mutant strains. Mutant B. subtilis PGA6‐2 stably produces high levels of γ‐PGA in medium A without supplementation of extra glutamic acid or ammonium chloride. The mutant B. subtilis PGA 6‐2 is not only a γ ‐PGA producer, but it is also a candidate for the genetic and metabolic engineering of γ ‐PGA production. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Год издания: 2010
Авторы: Chuan‐Mei Yeh, Jyh‐Perng Wang, Shih‐Ching Lo, Wen‐Chia Chan, Ming‐Yi Lin
Издательство: American Chemical Society
Источник: Biotechnology Progress
Ключевые слова: Biopolymer Synthesis and Applications, Cancer Research and Treatments, Polyamine Metabolism and Applications
Другие ссылки: Biotechnology Progress (HTML)
PubMed (HTML)