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Equilibrium andKinetic Analysis ofNucleotide Binding tothe DEAD-Box RNAHelicase DbpA Miguel A. TalaveraEnrique M. De La Cruz2004 год

Equilibrium and Kinetic Analysis of Nucleotide Binding to the DEAD-Box RNA Helicase DbpA
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Аннотация: The Escherichia coli DEAD-box protein A (DbpA) is an RNA helicase that utilizes the energy from ATP binding and hydrolysis to facilitate structural rearrangements of rRNA. We have used the fluorescent nucleotide analogues, mantADP and mantATP, to measure the equilibrium binding affinity and kinetic mechanism of nucleotide binding to DbpA in the absence of RNA. Binding generates an enhancement in mant-nucleotide fluorescence and a corresponding reduction in intrinsic DbpA fluorescence, consistent with fluorescence resonance energy transfer (FRET) from DbpA tryptophan(s) to bound nucleotides. Fluorescent modification does not significantly interfere with the affinities and kinetics of nucleotide binding. Different energy transfer efficiencies between DbpA-mantATP and DbpA-mantADP complexes suggest that DbpA adopts nucleotide-dependent conformations. ADP binds (Kd ∼ 50 μM at 22 °C) 4−7 times more tightly than ATP (Kd ∼ 400 μM at 22 °C). Both nucleotides bind with relatively temperature-independent association rate constants (∼1−3 μM-1 s-1) that are much lower than predicted for a diffusion-limited reaction. Differences in the binding affinities are dictated primarily by the dissociation rate constants. ADP binding occurs with a positive change in the heat capacity, presumably reflecting a nucleotide-induced conformational rearrangement of DbpA. At low temperatures (<22 °C), the binding free energies are dominated by favorable enthalpic and unfavorable entropic contributions. At physiological temperatures (>22 °C), ADP binding occurs with positive entropy changes. We favor a mechanism in which ADP binding increases the conformational flexibility and dynamics of DbpA.
Год издания: 2004
Авторы: Miguel A. Talavera, Enrique M. De La Cruz
Издательство: American Chemical Society
Источник: Biochemistry
Ключевые слова: RNA and protein synthesis mechanisms, RNA Research and Splicing, DNA and Nucleic Acid Chemistry
Другие ссылки: Biochemistry (HTML)
PubMed (HTML)