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Desmosome assembly inMDCK epithelial cellsdoes not require thepresence offunctionalmicrotubules Manijeh PasdarZhi LiKathleen A. Krzeminski1992 год

Desmosome assembly in MDCK epithelial cells does not require the presence of functional microtubules
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Аннотация: Abstract Desmosomes, complex multisubunit structures that assemble at sites of cell‐cell contact, are important components of the epithelial junctional complex. Desmosome assembly requires the coordinated interaction at the plasma membrane of at least 8 cytoplasmic and integral membrane proteins organized into two structurally and functionally distinct domains, the cytoplasmic plaque and membrane core. Previous studies (Pasdar et al., J. Cell Biol., 113:645–655) provided evidence that cytokeratin filaments and microtubules may regulate transfer and assembly of cytoplasmic plaque and membrane core proteins, respectively. To determine directly the role of microtubules in these processes, Madin‐Darby canine kidney (MDCK) cells were treated with nocodazole or colchicine to disrupt the microtubular network. Biochemical analysis of the different components of the cytoplasmic plaque and membrane core domains revealed little or no effect of nocodazole or colchicine on the kinetics of synthesis, post‐translational modifications, transfer of proteins to the plasma membrane or their metabolic stability in the presence or absence of cell‐cell contact. Likewise, immunofluorescence analysis of desmosome formation demonstratedan apparently normal desmosome assembly in the presence of nocodazole or colchicine upon induction of cell‐cell contact. These results indicate that an intact microtubular network is not necessary for the processing or transport of the desmosomal membrane core glycoproteins to the plasma membrane in the absence or presence of cell‐cell contact. Furthermore, the integration of the cytoplasmic plaque and membrane core domains induced by cell‐cell contact at the plasma membranes of adjacent cells does not require the presence of functional microtubules. © 1992 Wiley‐Liss, Inc.
Год издания: 1992
Авторы: Manijeh Pasdar, Zhi Li, Kathleen A. Krzeminski
Издательство: Wiley
Источник: Cell Motility and the Cytoskeleton
Ключевые слова: Microtubule and mitosis dynamics, Hippo pathway signaling and YAP/TAZ, Wnt/β-catenin signaling in development and cancer
Другие ссылки: Cell Motility and the Cytoskeleton (HTML)
PubMed (HTML)