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Growth of humanosteoblast-like cellson alkanethiol ongold self-assembledmonolayers: Theeffect of surfacechemistry Colin A. ScotchfordElaine CooperGraham J. Leggett1998 год

Growth of human osteoblast-like cells on alkanethiol on gold self-assembled monolayers: The effect of surface chemistry
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Аннотация: Primary human osteoblasts were cultured on self-assembled monolayers (SAMs) of alkylthiols on gold with carboxylic acid and methyl termini, and the kinetics of cell attachment and proliferation were measured. Over 90 min approximately twice as many cells attached to carboxylic-acid-terminated monolayers as attached to methyl-terminated monolayers. After 24 h the number of cells attached to carboxylic-acid-terminated monolayers was ten times that attached to the methyl-terminated monolayers. Cell morphology and cytoskeletal actin organization also were found to be different. Osteoblasts were cultured on SAMs that were patterned by photolithographic techniques. Cells attached almost exclusively to carboxylic-acid-functionalized areas of the patterned surfaces, leaving methyl-functionalized regions bare. The patterns strongly influenced the morphology of the attached cells. After 24 h, cells were observed to bridge between carboxylic-acid-terminated regions separated by 75 μm, methyl-terminated regions but not those separated by 150 μm methyl-terminated regions. After 6 days in culture osteoblasts formed multilayers on the carboxylic-acid-terminated regions of the pattern. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 41, 431–442, 1998.
Год издания: 1998
Авторы: Colin A. Scotchford, Elaine Cooper, Graham J. Leggett, S. Downes
Издательство: Wiley
Источник: Journal of Biomedical Materials Research
Ключевые слова: Polymer Surface Interaction Studies, Molecular Junctions and Nanostructures, Advanced biosensing and bioanalysis techniques
Другие ссылки: Journal of Biomedical Materials Research (HTML)
PubMed (HTML)