Аннотация:The CDC13 gene has previously been implicated in the maintenance of telomere integrity in Saccharomyces cerevisiae . With the use of two classes of mutations, here it is shown that CDC13 has two discrete roles at the telomere. The cdc13-2 est mutation perturbs a function required in vivo for telomerase regulation but not in vitro for enzyme activity, whereas cdc13-1 ts defines a separate essential role at the telomere. In vitro, purified Cdc13p binds to single-strand yeast telomeric DNA. Therefore, Cdc13p is a telomere-binding protein required to protect the telomere and mediate access of telomerase to the chromosomal terminus.
Издательство:American Association for the Advancement of Science
Источник:Science
Ключевые слова:Telomeres, Telomerase, and Senescence, Genetics, Aging, and Longevity in Model Organisms, bioluminescence and chemiluminescence research
