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High-ThroughputSequencing of RNASilencing-AssociatedSmall RNAs in Olive(Olea europaea L.) Livia DonaireLaia PedrolaRaúl de la Rosa2011 год

High-Throughput Sequencing of RNA Silencing-Associated Small RNAs in Olive (Olea europaea L.)
статья из журнала

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Аннотация: Small RNAs (sRNAs) of 20 to 25 nucleotides (nt) in length maintain genome integrity and control gene expression in a multitude of developmental and physiological processes. Despite RNA silencing has been primarily studied in model plants, the advent of high-throughput sequencing technologies has enabled profiling of the sRNA component of more than 40 plant species. Here, we used deep sequencing and molecular methods to report the first inventory of sRNAs in olive (Olea europaea L.). sRNA libraries prepared from juvenile and adult shoots revealed that the 24-nt class dominates the sRNA transcriptome and atypically accumulates to levels never seen in other plant species, suggesting an active role of heterochromatin silencing in the maintenance and integrity of its large genome. A total of 18 known miRNA families were identified in the libraries. Also, 5 other sRNAs derived from potential hairpin-like precursors remain as plausible miRNA candidates. RNA blots confirmed miRNA expression and suggested tissue- and/or developmental-specific expression patterns. Target mRNAs of conserved miRNAs were computationally predicted among the olive cDNA collection and experimentally validated through endonucleolytic cleavage assays. Finally, we use expression data to uncover genetic components of the miR156, miR172 and miR390/TAS3-derived trans-acting small interfering RNA (tasiRNA) regulatory nodes, suggesting that these interactive networks controlling developmental transitions are fully operational in olive.
Год издания: 2011
Авторы: Livia Donaire, Laia Pedrola, Raúl de la Rosa, César Llave
Издательство: Public Library of Science
Источник: PLoS ONE
Ключевые слова: Plant Molecular Biology Research, Chromosomal and Genetic Variations, Genomics and Phylogenetic Studies
Другие ссылки: PLoS ONE (PDF)
PLoS ONE (HTML)
DOAJ (DOAJ: Directory of Open Access Journals) (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
DIGITAL.CSIC (Spanish National Research Council (CSIC)) (PDF)
DIGITAL.CSIC (Spanish National Research Council (CSIC)) (HTML)
PubMed Central (HTML)
PubMed (HTML)