1. Главная
  2. Ресурсы
  3. Библиотечный поиск
A NovelSuperoxide-producingNAD(P)H Oxidase inKidney Akira ShioseJunya KurodaKazuhiko Tsuruya2001 год

A Novel Superoxide-producing NAD(P)H Oxidase in Kidney
статья из журнала

Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: During phagocytosis, gp91 phox , the catalytic subunit of the phagocyte NADPH oxidase, becomes activated to produce superoxide, a precursor of microbicidal oxidants. Currently increasing evidence suggests that nonphagocytic cells contain similar superoxide-producing oxidases, which are proposed to play crucial roles in various events such as cell proliferation and oxygen sensing for erythropoiesis. Here we describe the cloning of human cDNA that encodes a novel NAD(P)H oxidase, designated NOX4. The NOX4 protein of 578 amino acids exhibits 39% identity to gp91 phox with special conservation in membrane-spanning regions and binding sites for heme, FAD, and NAD(P)H, indicative of its function as a superoxide-producing NAD(P)H oxidase. The membrane fraction of kidney-derived human embryonic kidney (HEK) 293 cells, expressing NOX4, exhibits NADH- and NADPH-dependent superoxide-producing activities, both of which are inhibited by diphenylene iodonium, an agent known to block oxygen sensing, and decreased in cells expressing antisense NOX4 mRNA. The human NOX4 gene, comprising 18 exons, is located on chromosome 11q14.2-q21, and its expression is almost exclusively restricted to adult and fetal kidneys. In human renal cortex, high amounts of the NOX4 protein are present in distal tubular cells, which reside near erythropoietin-producing cells. In addition, overexpression of NOX4 in cultured cells leads to increased superoxide production and decreased rate of growth. The present findings thus suggest that the novel NAD(P)H oxidase NOX4 may serve as an oxygen sensor and/or a regulator of cell growth in kidney.
Год издания: 2001
Авторы: Akira Shiose, Junya Kuroda, Kazuhiko Tsuruya, Momoki Hirai, Hideki Hirakata, Seiji Naito, Masahira Hattori, Yoshiyuki Sakaki, Hideki Sumimoto
Издательство: Elsevier BV
Источник: Journal of Biological Chemistry
Ключевые слова: Neonatal Health and Biochemistry, Nitric Oxide and Endothelin Effects, Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Другие ссылки: Journal of Biological Chemistry (PDF)
Journal of Biological Chemistry (HTML)
PubMed (HTML)