1. Главная
  2. Ресурсы
  3. Библиотечный поиск
Microscopic-ObservationDrug-SusceptibilityAssay for theDiagnosis of TB David MooreCarlton A. EvansRobert H. Gilman2006 год

Microscopic-Observation Drug-Susceptibility Assay for the Diagnosis of TB
статья из журнала

Полный текстСтраница публикацииПубликация в OpenAlex
Аннотация: New diagnostic tools are urgently needed to interrupt the transmission of tuberculosis and multidrug-resistant tuberculosis. Rapid, sensitive detection of tuberculosis and multidrug-resistant tuberculosis in sputum has been demonstrated in proof-of-principle studies of the microscopic-observation drug-susceptibility (MODS) assay, in which broth cultures are examined microscopically to detect characteristic growth.In an operational setting in Peru, we investigated the performance of the MODS assay for culture and drug-susceptibility testing in three target groups: unselected patients with suspected tuberculosis, prescreened patients at high risk for tuberculosis or multidrug-resistant tuberculosis, and unselected hospitalized patients infected with the human immunodeficiency virus. We compared the MODS assay head-to-head with two reference methods: automated mycobacterial culture and culture on Löwenstein-Jensen medium with the proportion method.Of 3760 sputum samples, 401 (10.7%) yielded cultures positive for Mycobacterium tuberculosis. Sensitivity of detection was 97.8% for MODS culture, 89.0% for automated mycobacterial culture, and 84.0% for Löwenstein-Jensen culture (P<0.001); the median time to culture positivity was 7 days, 13 days, and 26 days, respectively (P<0.001), and the median time to the results of susceptibility tests was 7 days, 22 days, and 68 days, respectively. The incremental benefit of a second MODS culture was minimal, particularly in patients at high risk for tuberculosis or multidrug-resistant tuberculosis. Agreement between MODS and the reference standard for susceptibility was 100% for rifampin, 97% for isoniazid, 99% for rifampin and isoniazid (combined results for multidrug resistance), 95% for ethambutol, and 92% for streptomycin (kappa values, 1.0, 0.89, 0.93, 0.71, and 0.72, respectively).A single MODS culture of a sputum sample offers more rapid and sensitive detection of tuberculosis and multidrug-resistant tuberculosis than the existing gold-standard methods used.
Год издания: 2006
Авторы: David Moore, Carlton A. Evans, Robert H. Gilman, Luz Caviedes, Jorge Coronel, Aldo Vivar, Eduardo Sánchez, Yvette Piñedo, Juan Carlos Saravia, Cayo Salazar, Richard A. Oberhelman, Maria-Graciela Hollm-Delgado, Doris LaChira, A. Roderick Escombe, Jon S. Friedland
Издательство: Massachusetts Medical Society
Источник: New England Journal of Medicine
Ключевые слова: Tuberculosis Research and Epidemiology, Mycobacterium research and diagnosis, Bacterial Identification and Susceptibility Testing
Другие ссылки: New England Journal of Medicine (HTML)
Europe PMC (PubMed Central) (PDF)
Europe PMC (PubMed Central) (HTML)
LSHTM Research Online (London School of Hygiene & Tropical Medicine) (HTML)
PubMed Central (HTML)
PubMed (HTML)