Аннотация:SPECIFIC AIMSThis study was designed to investigate whether supplementation with CoQ10 protects DNA against oxidative damage in lymphocytes oxidized in vitro with compounds inducing different oxidizing profiles; DNA recovery from oxidative damage was also studied. The hypothesis that CoQ10 supplementation might modulate the repair enzyme activity will be tested on whole cell extract lymphocytes.PRINCIPAL FINDINGS1. Coenzyme Q10 in vitro enrichment inhibits the formation of DNA strand breaks induced by atmospheric oxygenThe incubation of lymphocytes under atmospheric oxygen leads to a formation of DNA strand breaks (SBs) detectable within the first hour of exposure. Both ubiquinol-10 and ubiquinone-10 are able to prevent DNA SB formation, the enriched lymphocytes resulting in less damage and exhibiting a faster DNA repair rate than that of nonenriched control lymphocytes (Fig. 1a⤻ ). Conversely, the ubiquinol-10 or ubiquinone-10 neither prevents the endogenous formation of oxidized purine and pyrimidine ba...
