Аннотация:The Golden Retriever Muscular Dystrophy (GRMD), is a degenerative myopathy caused by the absence of a protein, dystrophin, genetically homologous to the Duchenne Muscular Dystrophy that affects humans, so these dogs are considered experimental models for studies that seek treatments for this progressive disease with no cure.In this work, we selected seven different medications, including: Sildenafil, Ursodeoxycholic Acid, Acetylcysteine, Losartan Potassium, Mycophenolate mofetil, Thalidomide and Diltiazem concomitantly forming a cocktail of drugs in order to treat the deleterious effects of muscular dystrophy, thereby providing a better quality and survival for patients dystrophic.Each drug was chosen for its action: sildenafil, increasing nitric oxide; ursodeoxycholic acid, anti-inflammatory; acetylcysteine, an antioxidant; losartan, antifibrotic and cardioprotective; mycophenolate mofetil, an immunosuppressant; thalidomide, antiTNF; Diltiazem, calcium channel blocker.For the experiment, 6 male dogs were utilized GRMD, and two control and four experimental.Treatment started with 80 days to 10 months of age.The animals were evaluated at different times with serum levels, basic histology, immunohistochemistry and PCR.After the evaluations, we conclude that the cocktail of drugs, no clinical improvement in the experimental group.The dosage of leukocytes, ALT, CK and NFkβ and TGFβ1 expression was lower in the experimental group compared with the control group, suggesting an improvement in the inflammatory process and delayed progression of muscular dystrophy in the experimental group.
