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Cyclin A1 promoterhypermethylation inhumanpapillomavirus-associated cervical cancer Nakarin KitkumthornPattamawadee YanatatsanajitSorapop Kiatpongsan2006 год

Cyclin A1 promoter hypermethylation in human papillomavirus-associated cervical cancer
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Аннотация: Abstract Background The aim of this study was to evaluate epigenetic status of cyclin A1 in human papillomavirus-associated cervical cancer. Y. Tokumaru et al ., Cancer Res 64 , 5982-7 (Sep 1, 2004)demonstrated in head and neck squamous-cell cancer an inverse correlation between cyclin A1 promoter hypermethylation and TP53 mutation. Human papillomavirus-associated cervical cancer, however, is deprived of TP53 function by a different mechanism. Therefore, it was of interest to investigate the epigenetic alterations during multistep cervical cancer development. Methods In this study, we performed duplex methylation-specific PCR and reverse transcriptase PCR on several cervical cancer cell lines and microdissected cervical cancers. Furthermore, the incidence of cyclin A1 methylation was studied in 43 samples of white blood cells, 25 normal cervices, and 24, 5 and 30 human papillomavirus-associated premalignant, microinvasive and invasive cervical lesions, respectively. Results We demonstrated cyclin A1 methylation to be commonly found in cervical cancer, both in vitro and in vivo, with its physiological role being to decrease gene expression. More important, this study demonstrated that not only is cyclin A1 promoter hypermethylation strikingly common in cervical cancer, but is also specific to the invasive phenotype in comparison with other histopathological stages during multistep carcinogenesis. None of the normal cells and low-grade squamous intraepithelial lesions exhibited methylation. In contrast, 36.6%, 60% and 93.3% of high-grade squamous intraepithelial lesions, microinvasive and invasive cancers, respectively, showed methylation. Conclusion This methylation study indicated that cyclin A1 is a potential tumor marker for early diagnosis of invasive cervical cancer.
Год издания: 2006
Авторы: Nakarin Kitkumthorn, Pattamawadee Yanatatsanajit, Sorapop Kiatpongsan, Chureerat Phokaew, Surang Triratanachat, Prasert Trivijitsilp, Wichai Termrungruanglert, Damrong Tresukosol, Somchai Niruthisard, Apiwat Mutirangura
Издательство: BioMed Central
Источник: BMC Cancer
Ключевые слова: Epigenetics and DNA Methylation, Cervical Cancer and HPV Research, Cancer-related gene regulation
Другие ссылки: BMC Cancer (PDF)
BMC Cancer (HTML)
PubMed Central (HTML)
PubMed (HTML)