Аннотация:The immunobead test (IBT) is an excellent test for initial sperm antibody screening and evaluation but is impractical when used to quantitate antibody levels using a twofold dilution series. The aim of this study, therefore, was to develop a relatively simple radioimmunobinding assay that would allow quantitation of sperm antibodies of IgG immunoglobulin class, which predominate in male sera. [125I]-Protein G was chosen as the radioligand because it binds to all IgG subclasses, but not to IgA or IgM. The results of the investigation indicate that the [125I]-protein G assay (PGA) allows efficient quantitation of sperm antibodies, as evidenced by a highly significant (P less than .0001) correlation (Spearman's, Rs = 0.94) between the PGA results and IgG-IBT titres.
