Аннотация:Biologists can take beautiful pictures of whole cells and, at the other extreme, outline the molecular structure of single proteins. But they have not had a good way to look at midsized objects within a cell—organelles in the range of 5 to 10 nanometers, such as actin filaments that cleave a cell in half or the protein-packaging Golgi apparatus. Now, researchers have opened a window on such structures with a technique called cryoelectron tomography (cryo-ET), which combines flash-freezing of whole cells with automated electron scanning to create three- dimensional images of intact organelles.
