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In Vivo Expression ofGenetic InformationfromPhosphoramidate–DNA :научное издание Nguyen HoaiAbramov MikhailEremeeva Elena2020 год

In Vivo Expression of Genetic Information from Phosphoramidate–DNA : научное издание
статья из журнала

Дополнительные ссылки: eLibrary
База данных: Каталог библиотеки СФУ
Библиографическое описание: In Vivo Expression of Genetic Information from Phosphoramidate–DNA : научное издание / H. Nguyen, M. Abramov, E. Eremeeva, P. Herdewijn. - Текст : непосредственный // ChemBioChem. - 2020. - Т. 21, № 1-2. - P. 272-278. - ISSN 1439-4227. - ISSN 1439-7633, DOI 10.1002/cbic.201900712.
Аннотация: <jats:title>Abstract</jats:title><jats:p>Chemically modified genes and genomes with customized properties will become a valuable tool in numerous fields, including synthetic biology, biotechnology, and medicine. These genetic materials are meant to store and exchange information with DNA and RNA while tuning their functionality. Herein, we outline the development of an alternative genetic system carrying phosphoramidate linkages that successfully propagates genetic information in bacteria and at the same time is labile to acidic conditions. The P3′→N5′ phosphoramidate-containing DNA (PN-DNA) was enzymatically synthesized by using 5′-amino-2′,5′-deoxycytidine 5′-<jats:italic>N</jats:italic>-triphosphates (NH-dCTPs) as substrates for DNA polymerases and employed to encode antibiotic resistance in <jats:italic>Escherichia coli</jats:italic>. The resulting PN-DNA can be efficiently destroyed by mild acidic conditions, whereas an unmodified counterpart remains intact. A cloning strategy was proposed for assembling modified fragments into a genome. This method can be of interest to scientists working in the field of orthogonal nucleic acid genes and genomes.</jats:p>
Год издания: 2020
Авторы: Nguyen Hoai , Abramov Mikhail , Eremeeva Elena , Herdewijn Piet
Издательство: Wiley Interscience
Источник: ChemBioChem
Выпуск: Т. 21, № 1-2
Номера страниц: 272-278
Держатель оригинала документа: KU Leuven Rega Institute for Medical Research Medicinal Chemistry Herestraat 49 Box 1041 3000 Leuven Belgium
Количество экземпляров:
Твёрдая копия издания отсутствует в фонде библиотеки

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