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The Network ofReplication,Transcription, andReverse Transcriptionof a SyntheticGenetic Cassette :научное издание Yang HuiEremeeva ElenaAbramov Mikhail2021 год

The Network of Replication, Transcription, and Reverse Transcription of a Synthetic Genetic Cassette : научное издание
статья из журнала

Дополнительные ссылки: eLibrary
База данных: Каталог библиотеки СФУ
Библиографическое описание: The Network of Replication, Transcription, and Reverse Transcription of a Synthetic Genetic Cassette : научное издание / H. Yang, E. Eremeeva, M. Abramov, P. Herdewijn. - Текст : непосредственный // Angewandte Chemie International Edition. - 2021. - Т. 60, № 8. - P. 4175-4182. - ISSN 1433-7851. - ISSN 1521-3773, DOI 10.1002/anie.202011887.
Аннотация: <jats:title>Abstract</jats:title><jats:p>Synthetic nucleic acids, with four non-canonical nucleobases, can function as genetic materials. A comprehensive analysis of PCR amplification, transcription, reverse transcription, and cloning was done to screen for alternative genetic monomers. A small library of six modified nucleobases was selected: the modified 2′-deoxyribonucleoside (dZTPs) and ribonucleoside (rZTPs) triphosphates of 7-deaza-adenine, 5-chlorouracil, 7-deaza-guanine or inosine together with 5-fluorocytosine or 5-bromocytosine. The fragments composed of one to four modified nucleotides (denoted as DZA) have been successfully recognized and transcribed to natural or modified RNA (denoted as RZA) by T7 RNA polymerase. The fully modified RZA fragment could be reverse transcribed and then amplified in the presence of various dZTPs. Noticeably, modified fragments could function as genetic templates in vivo by encoding the 678 base pair gene of a fluorescent protein in bacteria. These results demonstrate the existence of a fully simulated genetic circuit that uses synthetic materials.</jats:p>
Год издания: 2021
Авторы: Yang Hui , Eremeeva Elena , Abramov Mikhail , Herdewijn Piet
Издательство: Wiley Interscience
Источник: Angewandte Chemie International Edition
Выпуск: Т. 60, № 8
Номера страниц: 4175-4182
Держатель оригинала документа: Medicinal Chemistry Rega Institute for Medical Research KU Leuven Herestraat 49, Box-1041 3000 Leuven Belgium
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