Библиографическое описание:The Effects of Thorium-232 on the Bioluminescent Enzymatic System and Radioprotective Activity of Humic Substances : научное издание / T. V. Rozhko [et al.]. - Текст : непосредственный // Biophysics. - 2024. - Т. 69, № 3. - P. 370-379. - The study was carried out within the framework of the State assignment of the Ministry of Science and Higher Education of the Russian Federation, project no. FWES-2024-0018. The work was partially supported by the Russian Science Foundation, project no. 23-26-10018 and the Krasnoyarsk Regional Science Foundation, Forecasting the reaction of farm animals to low-intensity radiation and the use of radioprotectors. Express bioluminescent screening of radiobiological effects project. - ISSN 0006-3509. - ISSN 1555-6654, DOI 10.1134/S0006350924700453.
Аннотация:The study of low-dose effects is relevant in connection with the expansion of areas with anthropogenic load. Thorium is one of the background-forming elements in natural ecosystems, its content can increase in the environment during the extraction of natural resources and the operation of thermal power plants. Of particular interest are the bioeffects of thorium in the presence of humic substances, natural detoxifiers. The aim of this study was to evaluate the neutralizing effect of humic substances under conditions of low-dose exposure to thorium-232 (<0.04 Gy). A bioluminescent system of enzymatic reactions, including bacterial luciferase and NAD(P)H:FMN-oxidoreductase, was used as a model biological object. Activation of bioluminescence at the initial stage of thorium-232 exposure (up to 50 min) and an increase in the content of reactive oxygen species after 50 min of exposure were observed. The relationship between the intensity of bioluminescence and the content of reactive oxygen species (correlation coefficient of –0.86) was shown. The presence of humic substances neutralized the activating effect of thorium and reduced the content of reactive oxygen species to a control level. The effect of humic substances on the rate of the NADH-dependent enzymatic redox process has been demonstrated. The results we obtained contribute to the understanding of the molecular mechanisms of thorium-232 exposure and the neutralization of its effect on enzymatic processes.
Держатель оригинала документа:Baikal Museum, Siberian Branch, Russian Academy of Sciences, Institute of Biophysics, Krasnoyarsk Scientific Center Federal Research Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk State Medical University named after Professor V.F. Voyno-Yasenetskii, Ministry of Health of the Russian Federation, Siberian Federal University, Tomsk Polytechnic University
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