Библиографическое описание:Effects of Selenite Ions on a Luminescence Enzymatic System : научное издание / Ekaterina S. Sushko, Andrei V. Zenkov, Nadezhda S. Kudryasheva. - Текст : непосредственный // Eurasian Journal of Chemistry. - 2024. - Т. 29, № 3. - P. 65-71. - This research was funded by the Russian Science Foundation and Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science (Grant No. 24-24-20001). Enzyme purification was supported by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation, project FWES-2024-0018. - ISSN 2959-0663. - ISSN 2959-0671, DOI 10.31489/2959-0663/3-24-6.
Аннотация:This paper elucidates biochemical and physicochemical aspects of toxicity of a redox-active compound in organisms. Sodium selenite (Na<sub>2</sub>SeO<sub>3</sub>) was chosen as a model redox-active compound; a coupled enzymatic system from luminous marine bacteria was applied to imitate a biochemical process. We demonstrated that Na<sub>2</sub>SeO<sub>3 </sub>suppressed bioluminescence of the enzyme system; the effective inhibition concentration was 10<sup>-2 </sup>M. Besides that, Na<sub>2</sub>SeO<sub>3</sub> decreased the content of reactive oxygen species (ROS) in aqueous solutions (enzyme-free media) at >10<sup>-3 </sup>M. Addition of enzymes and their substrates to these solutions enhanced this decrease. Correlations between dependencies of the ROS content and bioluminescence intensity on the concentration of Na<sub>2</sub>SeO<sub>3</sub> were positive and high, confirming the ROS involvement in the bioluminescence suppression in Na<sub>2</sub>SeO<sub>3</sub> solutions. Hence, we observed the disturbance of the native biochemical oxidative functions of dissolved oxygen derivatives under exposure to redox-active toxicants. The effects of Na<sub>2</sub>SeO<sub>3</sub> on the bacterial enzyme system should be further compared with those on bacterial cells, which are traditionally used as a toxicity bioassay. Additionally, the use of natural microorganisms is perspective due to their ability of redox transformation of toxic selenium oxoanions to elemental selenium. This ability is important as it provides: (1) natural detoxification of water ecosystems, and (2) biosynthesis of selenium nanoparticles.
