Библиографическое описание:Visualization of Brain Tumors with Infrared-Labeled Aptamers for Fluorescence-Guided Surgery : научное издание / G. G. Zamay [et al.]. - Текст : непосредственный // Journal of the American Chemical Society. - 2024. - Т. 146, № 36. - P. 24989-25004. - ISSN 0002-7863. - ISSN 1520-5126, DOI 10.1021/jacs.4c06716.
Аннотация:Gliomas remain challenging brain tumors to treat due to their infiltrative nature. Accurately identifying tumor boundaries during surgery is crucial for successful resection. This study introduces an innovative intraoperative visualization method utilizing surgical fluorescence microscopy to precisely locate tumor cell dissemination. Here, the focus is on the development of a novel contrasting agent (IR-Glint) for intraoperative visualization of human glial tumors comprising infrared-labeled Glint aptamers. The specificity of IR-Glint is assessed using flow cytometry and microscopy on primary cell cultures. In vivo effectiveness is studied on mouse and rabbit models, employing orthotopic xenotransplantation of human brain gliomas with various imaging techniques, including PET/CT, in vivo fluorescence visualization, confocal laser scanning, and surgical microscopy. The experiments validate the potential of IR-Glint for the intraoperative visualization of gliomas using infrared imaging. IR-Glint penetrates the blood-brain barrier and can be used for both intravenous and surface applications, allowing clear visualization of the tumor. The surface application directly to the brain reduces the dosage required and mitigates potential toxic effects on the patient. The research shows the potential of infrared dye-labeled aptamers for accurately visualizing glial tumors during brain surgery. This novel aptamer-assisted fluorescence-guided surgery (AptaFGS) may pave the way for future advancements in the field of neurosurgery.
Держатель оригинала документа:Aptamerlab LLC, Krasnoyarsk 660042, Russia, Department of Chemistry and Biomolecular Sciences, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada, Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences”, Krasnoyarsk 660036, Russia, Federal Siberian Research Clinical Centre under the Federal Medical Biological Agency, Krasnoyarsk 660130, Russia, Kirensky Institute of Physics, Krasnoyarsk 660036, Russia, Krasnoyarsk Inter-District Ambulance Hospital Named after N.S. Karpovich, 17 Kurchatova, Krasnoyarsk 660062, Russia, Krasnoyarsk Regional Clinical Cancer Center, 16 1-ya Smolenskaya, Krasnoyarsk 660133, Russia, Krasnoyarsk Regional Pathology-Anatomic Bureau, Partizana Zheleznyaka, Krasnoyarsk 660022, Russia, Laboratory of Advanced Materials and Technology, Tomsk State University, Tomsk 634050, Russia, National Scientific Center of Marine Biology Far Eastern Branch of Russian Academy of Sciences, Prof. V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk 660022, Russia, School of Medicine and Life Sciences, Far Eastern Federal University, Vladivostok 690922, Russia
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