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The use ofbioluminescent enzymebioassay for theanalysis of humansaliva: Advantagesand disadvantages :научное издание… Kolenchukova Oksana A.Dedora Anastasia O.Stepanova Lyudmila V.2024 год

The use of bioluminescent enzyme bioassay for the analysis of human saliva: Advantages and disadvantages : научное издание
статья из журнала

Дополнительные ссылки: eLibrary
База данных: Каталог библиотеки СФУ
Библиографическое описание: The use of bioluminescent enzyme bioassay for the analysis of human saliva: Advantages and disadvantages : научное издание / Oksana A. Kolenchukova [et al.]. - Текст : непосредственный // Luminescence. - 2024. - Т. 39, № 5. - ISSN 1522-7235. - ISSN 1522-7243, DOI 10.1002/bio.4776.
Аннотация: <jats:title>Abstract</jats:title><jats:p>The purpose of the work was to find optimal conditions for bioluminescent enzymatic analysis of saliva (based on the use of NADH:FMN oxidoreductase + luciferase) and then to determine the biological effect of using bioluminescence assay of saliva to study the physiological state of the body under normal and pathological conditions. The saliva of snowboarders and students were studied in the “rest–training” model. The saliva of patients diagnosed with acute pharyngitis was examined in the “sick–healthy” model. Bioluminescence assay was performed with a lyophilized and immobilized bi-enzyme system using cuvette, plate, and portable luminometers. The concentrations of secretory immunoglobulin A (sIgA) and cortisol were determined by enzyme immunoassay, and the total protein content was measured by spectrophotometric method. The activity of the bioluminescent system enzymes increased as the amount and volume of saliva in the sample was decreased. The cuvette and plate luminometers were sensitive to changes in the luminescence intensity in saliva assay. Luminescence intensity correlated with the concentrations of sIgA and cortisol. The integrated bioluminescent index for saliva was reduced in the “rest–training” model and increased in the “sick–healthy” model. Thus, the non-invasive bioluminescent saliva analysis may be a promising tool for assessing the health of the population.</jats:p>
Год издания: 2024
Авторы: Kolenchukova Oksana A. , Dedora Anastasia O. , Stepanova Lyudmila V. , Kravchuk Vlada U. , Kratasyuk Valentina A.
Издательство: John Wiley & Sons
Источник: Luminescence
Выпуск: Т. 39, № 5
Держатель оригинала документа: Institute of Biophysics Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences” Krasnoyarsk Russia, Krasnoyarsk State Agrarian University Krasnoyarsk Russia, Research Institute of Medical Problems of the North Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences” Krasnoyarsk Russia, School of Fundamental Biology and Biotechnology Siberian Federal University Krasnoyarsk Russia
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