Three-Dimensional Printing of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] Biodegradable Scaffolds: Properties, In Vitro and In Vivo Evaluation : научное издание
Библиографическое описание:Three-Dimensional Printing of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] Biodegradable Scaffolds: Properties, In Vitro and In Vivo Evaluation : научное издание / Ekaterina I. Shishatskaya [et al.]. - Текст : непосредственный // International Journal of Molecular Sciences. - 2023. - Т.24, №16. - P. 12969. - ISSN 1661-6596. - ISSN 1422-0067, DOI 10.3390/ijms241612969.
Аннотация:<jats:p>The results of constructing 3D scaffolds from degradable poly(3-hydrosbutyrpate-co-3-hydroxyvalerate) using FDM technology and studying the structure, mechanical properties, biocompatibility in vitro, and osteoplastic properties in vivo are presented. In the process of obtaining granules, filaments, and scaffolds from the initial polymer material, a slight change in the crystallization and glass transition temperature and a noticeable decrease in molecular weight (by 40%) were registered. During the compression test, depending on the direction of load application (parallel or perpendicular to the layers of the scaffold), the 3D scaffolds had a Young’s modulus of 207.52 ± 19.12 and 241.34 ± 7.62 MPa and compressive stress tensile strength of 19.45 ± 2.10 and 22.43 ± 1.89 MPa, respectively. SEM, fluorescent staining with DAPI, and calorimetric MTT tests showed the high biological compatibility of scaffolds and active colonization by NIH 3T3 fibroblasts, which retained their metabolic activity for a long time (up to 10 days). The osteoplastic properties of the 3D scaffolds were studied in the segmental osteotomy test on a model defect in the diaphyseal zone of the femur in domestic Landrace pigs. X-ray and histological analysis confirmed the formation of fully mature bone tissue and complete restoration of the defect in 150 days of observation. The results allow us to conclude that the constructed resorbable 3D scaffolds are promising for bone grafting.</jats:p>
Источник:International Journal of Molecular Sciences
Выпуск:Т.24, №16
Номера страниц:12969
Держатель оригинала документа:Clinical Hospital “RZD-Medicine”, Lomonosov Street, 47, 660058 Krasnoyarsk, Russia, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok, 50/50, 660036 Krasnoyarsk, Russia, School of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodnyi Av. 79, 660041 Krasnoyarsk, Russia
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