1. Главная
  2. Ресурсы
  3. Библиотечный поиск
Implication ofstorage conditions onluminescence responsefromscpPhotobacteriumphosphoreum/scp infree and immobilizedform Ranjan RajeevAlamanova AzharSarangova Antonina2023 год

Implication of storage conditions on luminescence response from scpPhotobacterium phosphoreum/scp in free and immobilized form : научное издание
статья из журнала

Дополнительные ссылки: eLibrary
База данных: Каталог библиотеки СФУ
Библиографическое описание: Implication of storage conditions on luminescence response from scpPhotobacterium phosphoreum/scp in free and immobilized form : научное издание / R. R. Ranjan [et al.]. - Текст : непосредственный // Luminescence. - 2023. - Т. 38, № 6. - P. 717-721. - ISSN 1522-7235. - ISSN 1522-7243, DOI 10.1002/bio.4499.
Аннотация: <jats:title>Abstract</jats:title><jats:p>Bioluminescent bacteria in the form of a cell suspension for on-site hazard analysis are not suitable as <jats:italic>in vivo</jats:italic> luminescence in free cells fluctuates and may lead to erroneous results. Furthermore, the culture broth cannot be stored for long durations to continue sensing analytes as the luminescence ceases over time. Factors that affect luminescence response include growth dynamism, and ambient environmental conditions. The present study investigated the effect of storage conditions such as temperature (25 ± 2°C, room temperature; 4°C; and −20°C) and ambient aqueous environment (M1: sucrose, 1.02 M; M2, bioluminescent media [tryptone, 10 g L<jats:sup>−1</jats:sup>; NaCl, 28.5 g L<jats:sup>−1</jats:sup>; MgCl<jats:sub>2</jats:sub>.7H<jats:sub>2</jats:sub>O, 4.5 g L<jats:sup>−1</jats:sup>; CaCl<jats:sub>2</jats:sub>, 0.5 g L<jats:sup>−1</jats:sup>; KCl 0.5 g L<jats:sup>−1</jats:sup>; yeast extract, 1 g L<jats:sup>−1</jats:sup>; H<jats:sub>2</jats:sub>O, 1 L]; M3, bioluminescent media and 95% glycerol, 1:1 ratio) on the luminescence emission from the calcium alginate-immobilized <jats:styled-content style="fixed-case"><jats:italic>Photobacterium phosphoreum</jats:italic></jats:styled-content> (S<jats:sub>b</jats:sub>) against the cells in free suspension for an extended period. The results indicated that both the parameters that were undertaken markedly affected the luminescence. In the study, S<jats:sub>b</jats:sub> showed an enhanced luminescence emission than the control up to 18.5-fold and for a prolonged period which can be efficiently utilized for rapid biosensing of hazardous materials.</jats:p>
Год издания: 2023
Авторы: Ranjan Rajeev , Alamanova Azhar , Sarangova Antonina , Kratasyuk Valentina A.
Издательство: John Wiley & Sons
Источник: Luminescence
Выпуск: Т. 38, № 6
Номера страниц: 717-721
Держатель оригинала документа: Department of Biomedical Science, Acharya Narendra Dev College University of Delhi, Govindpuri, Kalkaji New Delhi India, Institute of Biophysics SB RAS Federal Research Center ‘Krasnoyarsk Science Center SB RAS’ Akademgorodok 50/50 Krasnoyarsk Russia, Laboratory of Bioluminescent Biotechnologies, Department of Biophysics, Institute of Fundamental Biology and Biotechnology Siberian Federal University 79 Svobodny Prospect Krasnoyarsk Russia
Количество экземпляров:
Твёрдая копия издания отсутствует в фонде библиотеки

Похожие публикации

The use ofbioluminescent enzymebioassay for theanalysis of humansaliva: Advantagesand disadvantages Kolenchukova Oksana A.Dedora Anastasia O.Stepanova Lyudmila V.2024 год

The use of bioluminescent enzyme bioassay for the analysis of human saliva: Advantages and… : научное издание

  • Kolenchukova Oksana A. и др.
  • 2024 год
Immobilization ofFireflyBioluminescentSystem: Developmentand Application ofReagents Esimbekova Elena N.Kirillova Maria A.Kratasyuk Valentina A.2023 год

Immobilization of Firefly Bioluminescent System: Development and Application of Reagents : статья из журнала

  • Esimbekova Elena N. и др.
  • 2023 год
SolvatochromicPhotoluminescentEffects inAll-InorganicManganese(II)-BasedPerovskites by HighlySelective Solvent… Xiao HuiDang PeipeiYun Xiaohan2021 год

Solvatochromic Photoluminescent Effects in All-Inorganic Manganese(II)-Based Perovskites by Highly… : научное издание

  • Xiao Hui и др.
  • 2021 год