Coupling of scpNAD/scp(P)H:scpFMN/scp-oxidoreductase and luciferase from luminous bacteria in a viscous medium: Finding the weakest link in the chain : научное издание

Sutormin Oleg S.; Nemtseva Elena V.; Gulnov Dmitry V.; Sukovatyi Lev A.; Tyrtyshnaya Yekaterina S.; Lisitsa Albert E.; Kratasyuk Valentina A.

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База данных: Каталог библиотеки СФУ

Библиографическое описание: Coupling of scpNAD/scp(P)H:scpFMN/scp-oxidoreductase and luciferase from luminous bacteria in a viscous medium: Finding the weakest link in the chain : научное издание / Oleg S. Sutormin [et al.]. - Текст : непосредственный // Photochemistry and Photobiology. - 2023. - № . - ISSN 0031-8655. - ISSN 1751-1097, DOI 10.1111/php.13845.

Аннотация: AbstractThe study aims at revealing the mechanisms of the viscous medium effects on the kinetic features of NAD(P)H:FMN-oxidoreductase from luminous bacteria (Red), which are exhibited in a single enzyme assay and in coupling with bacterial luciferase (BLuc). Different concentrations of glycerol and sucrose were used to vary the medium viscosity. The activity of Red, alone and in the presence of BLuc, was analyzed, as well as BLuc activity in the presence of Red, whereas in the absence of BLuc, the Red activity was suppressed in viscous medium, and in the presence of BLuc, the increase in Red activity was observed at low glycerol concentrations (5–20 wt%). The interaction of glycerol and sucrose with Red substrates FMN and NADH was studied using absorption spectroscopy and molecular dynamics. Glycerol was found to form hydrogen bonds with the phosphate groups of the substrates, unlike sucrose. A mechanism for the activation of Red in the presence of BLuc in glycerol solutions through the acceleration of FMN reoxidation was proposed. Thus, it was concluded that, under the conditions used, the weakest link of the coupled enzyme system BLuc-Red in viscous medium is the FMN concentration, which depends on Red activity and the medium viscosity.

Год издания: 2023

Авторы: Sutormin Oleg S. , Nemtseva Elena V. , Gulnov Dmitry V. , Sukovatyi Lev A. , Tyrtyshnaya Yekaterina S. , Lisitsa Albert E. , Kratasyuk Valentina A.

Издательство: American Society for Photobiology

Источник: Photochemistry and Photobiology

Держатель оригинала документа: Department of Biophysics Institute of Fundamental Biology and Biotechnology, Siberian Federal University Krasnoyarsk Russia, Department of Chemistry, Institute of Natural and Technical Sciences Surgut State University Surgut Russia, Photobiology Laboratory Institute of Biophysics, Russian Academy of Sciences Krasnoyarsk Russia

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Coupling of scpNAD/scp(P)H:scpFMN/scp-oxidoreductase and luciferase from luminous bacteria in a viscous medium: Finding the weakest link in the chain : научное издание
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