Biosynthesis of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from Different 4-Hydroxybutyrate Precursors by New Wild-Type Strain Cupriavidus necator IBP/SFU-1 : научное издание
Библиографическое описание:Biosynthesis of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from Different 4-Hydroxybutyrate Precursors by New Wild-Type Strain Cupriavidus necator IBP/SFU-1 : научное издание / Natalia O. Zhila [et al.]. - Текст : непосредственный // Processes. - 2023. - Т.11, №5. - P. 1423. - ISSN 2227-9717, DOI 10.3390/pr11051423.
Аннотация:<jats:p>The study addresses the growth of the new wild-type strain Cupriavidus necator IBP/SFU-1 and the synthesis of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB) on media containing fructose and three different precursors of 4HB (ε-caprolactone 1,4-butanediol and 1,6-hexanediol). It was found that ε-caprolactone is the best precursor for the synthesis of P(3HB-co-4HB) copolymers. By varying the concentration and number of doses of ε-caprolactone added into the bacterial culture, it was possible to find conditions that ensured the synthesis of P(3HB-co-4HB) copolymers with different contents of 4HB (from 3–5 to 22.4 mol.%). The physicochemical properties of the copolymers were investigated depending on the proportions of 4HB monomers. The effect of 4HB monomers was manifested in a certain decrease in the weight-average molecular weight (Mw) (272–353 kDa), number-average molecular weight (Mn) (47–67 kDa) of the samples, and an increase in polydispersity (5.09–6.71) compared with P(3HB). The crystallinity degree decreased with an increasing fraction of the 4HB units (from 72 to 59%, as the 4HB content increased from 0 to 22.4 mol.%). In addition, the increase in 4HB content affected the temperature parameters (melting point, glass transition temperature, crystallization temperature, and thermal degradation temperature).</jats:p>
Держатель оригинала документа:Basic Department of Biotechnology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia, Chemistry Engineering Centre, ITMO University, Kronverkskiy Prospekt, 49A, Saint Petersburg 197101, Russia, Department of Medical Biology, School of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodnyi Av., Krasnoyarsk 660041, Russia, Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, 50/50 Akademgorodok, Krasnoyarsk 660036, Russia
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