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Engineering potentlive attenuatedcoronavirus vaccinesby targetedinactivation of theimmune evasive viraldeubiquitinase Myeni Sebenzile K.Bredenbeek Peter J.Knaap Robert C. M.2023 год

Engineering potent live attenuated coronavirus vaccines by targeted inactivation of the immune evasive viral deubiquitinase
статья из журнала

Дополнительные ссылки: eLibrary
База данных: Каталог библиотеки СФУ
Библиографическое описание: Engineering potent live attenuated coronavirus vaccines by targeted inactivation of the immune evasive viral deubiquitinase : научное издание / Sebenzile K. Myeni [et al.]. - Текст : непосредственный // Nature Communications. - 2023. - Т. 14, № 1. - P. 1141. - ISSN 2041-1723, DOI 10.1038/s41467-023-36754-z.
Аннотация: <jats:title>Abstract</jats:title><jats:p>Coronaviruses express a papain-like protease (PLpro) that is required for replicase polyprotein maturation and also serves as a deubiquitinating enzyme (DUB). In this study, using a Middle East respiratory syndrome virus (MERS-CoV) PLpro modified virus in which the DUB is selectively inactivated, we show that the PLpro DUB is an important MERS-CoV interferon antagonist and virulence factor. Although the DUB-negative rMERS-CoV<jats:sub>MA</jats:sub> replicates robustly in the lungs of human dipeptidyl peptidase 4 knock-in (hDPP4 KI) mice, it does not cause clinical symptoms. Interestingly, a single intranasal vaccination with DUB-negative rMERS-CoV<jats:sub>MA</jats:sub> induces strong and sustained neutralizing antibody responses and sterilizing immunity after a lethal wt virus challenge. The survival of naïve animals also significantly increases when sera from animals vaccinated with the DUB-negative rMERS-CoV<jats:sub>MA</jats:sub> are passively transferred, prior to receiving a lethal virus dose. These data demonstrate that DUB-negative coronaviruses could be the basis of effective modified live attenuated vaccines.</jats:p>
Год издания: 2023
Авторы: Myeni Sebenzile K. , Bredenbeek Peter J. , Knaap Robert C. M. , Dalebout Tim J. , Morales Shessy Torres , Sidorov Igor A. , Linger Marissa E. , Oreshkova Nadia , van Zanen-Gerhardt Sophie , Zander Serge A. L. , Enjuanes Luis , Sola Isabel , Snijder Eric J. , Kikkert Marjolein
Источник: Nature Communications
Выпуск: Т. 14, № 1
Номера страниц: 1141
Держатель оригинала документа: Campus Universidad Autonoma de Madrid, Leiden University Medical Center
Количество экземпляров:
Твёрдая копия издания отсутствует в фонде библиотеки

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