Crystal structure of semi-synthetic obelin-v after calcium induced bioluminescence implies coelenteramine as the main reaction product : научное издание

Natashin Pavel V.; Eremeeva Elena V.; Shevtsov Mikhail B.; Kovaleva Margarita I.; Bukhdruker Sergey S.; Dmitrieva Daria A.; Gulnov Dmitry V.; Nemtseva Elena V.; Gordeliy Valentin I.; Mishin Alexey V.; Borshchevskiy Valentin I.; Vysotski Eugene S.

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База данных: Каталог библиотеки СФУ

Библиографическое описание: Crystal structure of semi-synthetic obelin-v after calcium induced bioluminescence implies coelenteramine as the main reaction product : научное издание / Pavel V. Natashin [et al.]. - Текст : непосредственный // Scientific Reports. - 2022. - Т.12, №1. - P. 19613. - ISSN 2045-2322, DOI 10.1038/s41598-022-24117-5.

Аннотация: AbstractCoelenterazine-v (CTZ-v), a synthetic vinylene-bridged π-extended derivative, is able to significantly alter bioluminescence spectra of different CTZ-dependent luciferases and photoproteins by shifting them towards longer wavelengths. However, Ca2+-regulated photoproteins activated with CTZ-v display very low bioluminescence activities that hampers its usage as a substrate of photoprotein bioluminescence. Here, we report the crystal structure of semi-synthetic Ca2+-discharged obelin-v bound with the reaction product determined at 2.1 Å resolution. Comparison of the crystal structure of Ca2+-discharged obelin-v with those of other obelins before and after bioluminescence reaction reveals no considerable changes in the overall structure. However, the drastic changes in CTZ-binding cavity are observed owing to the completely different reaction product, coelenteramine-v (CTM-v). Since CTM-v is certainly the main product of obelin-v bioluminescence and is considered to be a product of the “dark” pathway of dioxetanone intermediate decomposition, it explains the low bioluminescence activity of obelin and apparently of other photoproteins with CTZ-v.

Год издания: 2022

Авторы: Natashin Pavel V., Eremeeva Elena V., Shevtsov Mikhail B., Kovaleva Margarita I., Bukhdruker Sergey S., Dmitrieva Daria A., Gulnov Dmitry V., Nemtseva Elena V., Gordeliy Valentin I., Mishin Alexey V., Borshchevskiy Valentin I., Vysotski Eugene S.

Источник: Scientific Reports

Выпуск: Т.12, №1

Номера страниц: 19613

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Crystal structure of semi-synthetic obelin-v after calcium induced bioluminescence implies coelenteramine as the main reaction product : научное издание
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