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EnzymeInhibition-BasedAssay to Estimate theContribution ofFormulants to theEffect of CommercialPesticideFormulations Esimbekova Elena N.Kalyabina Valeriya P.Kopylova Kseniya V.2023 год

Enzyme Inhibition-Based Assay to Estimate the Contribution of Formulants to the Effect of Commercial Pesticide Formulations : научное издание
статья из журнала

Дополнительные ссылки: eLibrary
База данных: Каталог библиотеки СФУ
Библиографическое описание: Enzyme Inhibition-Based Assay to Estimate the Contribution of Formulants to the Effect of Commercial Pesticide Formulations : научное издание / Elena N. Esimbekova [et al.]. - Текст : непосредственный // International Journal of Molecular Sciences. - 2023. - Т. 24, № 3. - P. 2248. - ISSN 1661-6596. - ISSN 1422-0067.
Аннотация: Pesticides can affect the health of individual organisms and the function of the entire ecosystem. Therefore, thorough assessment of the risks associated with the use of pesticides is a high-priority task. An enzyme inhibition-based assay is used in this study as a convenient and quick tool to study the effects of pesticides at the molecular level. The contribution of formulants to toxicological properties of the pesticide formulations has been studied by analyzing effects of 7 active ingredients of pesticides (AIas) and 10 commercial formulations based on them (AIfs) on the function of a wide range of enzyme assay systems differing in complexity (single-, coupled, and three-enzyme assay systems). Results have been compared with the effects of AIas and AIfs on bioluminescence of the luminous bacterium Photobacterium phosphoreum. Mostly, AIfs produce a considerably stronger inhibitory effect on the activity of enzyme assay systems and bioluminescence of the luminous bacterium than AIas, which confirms the contribution of formulants to toxicological properties of the pesticide formulation. Results of the current study demonstrate that “inert” ingredients are not ecotoxicologically safe and can considerably augment the inhibitory effect of pesticide formulations; therefore, their use should be controlled more strictly. Circular dichroism and fluorescence spectra of the enzymes used for assays do not show any changes in the protein structure in the presence of commercial pesticide formulations during the assay procedure. This finding suggests that pesticides produce the inhibitory effect on enzymes through other mechanisms.
Год издания: 2023
Авторы: Esimbekova Elena N. , Kalyabina Valeriya P. , Kopylova Kseniya V. , Lonshakova-Mukina Victoria I. , Antashkevich Anna A. , Torgashina Irina G. , Lukyanenko Kirill A. , Nemtseva Elena V. , Kratasyuk Valentina A.
Издательство: Molecular Diversity Preservation International?, Basel
Источник: International Journal of Molecular Sciences
Выпуск: Т. 24, № 3
Номера страниц: 2248
Держатель оригинала документа: Federal Research Center “Krasnoyarsk Research?Center” of Siberian Branch of Russian Academy of Science,, Institute of Biophysics of Siberian Branch of Russian Academy of Science, Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science, Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Siberian Federal University
Количество экземпляров:
Твёрдая копия издания отсутствует в фонде библиотеки

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