Библиографическое описание:Monitoring of breast cancer progression via aptamer-based detection of circulating tumor cells in clinical blood samples : научное издание / Olga S. Kolovskaya [et al.]. - Текст : непосредственный // Frontiers in Molecular Biosciences. - 2023. - Т.10, №JUN. - P. 1184285. - ISSN 2296-889X, DOI 10.3389/fmolb.2023.1184285.
Аннотация:Introduction: Breast cancer (BC) diagnostics lack noninvasive methods and procedures for screening and monitoring disease dynamics. Admitted CellSearch® is used for fluid biopsy and capture of circulating tumor cells of only epithelial origin. Here we describe an RNA aptamer (MDA231) for detecting?BC cells in clinical samples, including blood. The MDA231 aptamer was originally selected against triple-negative breast cancer cell line MDA-MB-231 using cell- SELEX.?Methods: The aptamer structure in solution was predicted using mFold program and molecular dynamic simulations. The affinity and specificity of the evolved aptamers were evaluated by flow cytometry and laser scanning microscopy on clinical tissues from breast cancer patients. CTCs were isolated form the patients' blood using the developed method of aptamer-based magnetic separation. Breast cancer origin of CTCs was confirmed by cytological, RT-qPCR and?Immunocytochemical analyses.?Results: MDA231 can specifically recognize breast cancer cells in surgically resected tissues from patients with different molecular subtypes: triple-negative,Luminal A, and Luminal B, but not in benign tumors, lung cancer, glial tumor and healthy epithelial from lungs and breast. This RNA aptamer can identify cancer cells in complex cellular environments, including tumor biopsies (e.g., tumor tissues vs.?margins) and clinical blood samples (e.g., circulating tumor cells). Breast cancer origin of the aptamer-based magnetically separated CTCs has been proved by immunocytochemistry and mammaglobin mRNA expression.?Discussion: We suggest a simple, minimally-invasive breast cancer diagnostic method based on non-epithelial MDA231 aptamer-specific magnetic isolation of circulating tumor cells. Isolated cells are intact and can be utilized for molecular diagnostics purposes.?
Держатель оригинала документа:Federal Research Center “Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of?Science”, Institute for Analytical Instrumentation of the Russian Academy of?Sciences, Kirensky Institute of Physics, Krasnoyarsk Regional Pathology-?Anatomic Bureau, Krasnoyarsk Regional?Clinical Cancer Center Named After A.I. Kryzhanovsky, latform Discovery Sciences, Biology, Wave Life Sciences, Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University, Russian Scientific Center of?Roentgenoradiology, Saint Petersburg National Research Academic University of the Russian Academy of?Sciences, Siberian Federal University, Siberian?Physical Technical Institute, Tomsk State University, University of Iowa, University of Ottawa
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