Библиографическое описание:Low pH Stress Enhances Gluconic Acid Accumulation with Enzymatic Hydrolysate as Feedstock Using Gluconobacter oxydans : научное издание / L. L. Dai [et al.]. - Текст : непосредственный // Fermentation. - 2023. - Т. 9, № 3. - P. 278. - This research was funded by the financial support from the National Natural Science Foundation of China (31901270) and the Postgraduate Research & Practice Innovation Program of Jiangsu Province (SJCX22_0324), Qing Lan Project of Jiangsu Province, China. - ISSN 2311-5637, DOI 10.3390/fermentation9030278.
Аннотация:Gluconic acid has been increasingly in demand in recent years due to the wide applications in the food, healthcare and construction industries. Plant-derived biomass is rich in biopolymers that comprise glucose as the monomeric unit, which provide abundant feedstock for gluconic acid production. Gluconobacter oxydans can rapidly and incompletely oxidize glucose to gluconic acid and it is regarded as ideal industrial microorganism. Once glucose is depleted, the gluconic acid will be further bio-oxidized to 2-ketogluconic acid by Gluconobacter oxydans. The endpoint is difficult to be controlled, especially in an industrial fermentation process. In this study, it was found that the low pH environment (2.5~3.5) could limit the further metabolism of gluconic acid and that it resulted in a yield over 95%. Therefore, the low pH stress strategy for efficiently producing gluconic acid from biomass-derived glucose was put forward and investigated with enzymatic hydrolysate. As a result, 98.8 g/L gluconic acid with a yield of 96% could be obtained from concentrated corncob enzymatic hydrolysate that initially contained 100 g/L glucose with 1.4 g/L cells loading of Gluconobacter oxydans. In addition, the low pH stress strategy could effectively control end-point and decrease the risk of microbial contamination. Overall, this strategy provides a potential for industrial gluconic acid production from lignocellulosic materials
Держатель оригинала документа:Institute of Chemistry and Chemical Technology SB RAS, FRC KSC SB RAS, Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Jiangsu Province Key Laboratory of Biomass Energy and Materials, Institute of Chemical Industry of Forest Products, Chinese Academy of Forestry, School of Basic Medical Sciences and Forensic Medicine, Hangzhou Medical College
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