Focal adhesion alterations in G0-positive melanoma cells : научное издание

Esimbekova Alexandra R.; Palkina Nadezhda V.; Zinchenko Ivan S.; Belenyuk Vasiliy D.; Savchenko Andrey A.; Sergeeva Ekaterina Yu; Ruksha Tatiana G.

Дополнительные ссылки: eLibrary

База данных: Каталог библиотеки СФУ

Библиографическое описание: Focal adhesion alterations in G0-positive melanoma cells : научное издание / Alexandra R. Esimbekova [et al.]. - Текст : непосредственный // Cancer Medicine. - 2022. - № б/н. - P. 1-15. - ISSN 2045-7634, DOI 10.1002/cam4.5510.

Аннотация: Abstract?Background: Melanoma is a highly heterogeneous malignant tumor that exhibits various forms of drug resistance. Recently, reversal transition of cancer cells to the G0 phase of the cell cycle under the influence of therapeutic drugs has been identified as an event associated with tumor dissemination. In the present study, we investigated the ability of chemotherapeutic agent dacarbazine to induce a transition of melanoma cells to the G0 phase as a mechanism of chemoresistance.??Methods: We used the flow cytometry to analyze cell distribution within cell cycle phases after dacarbazine treatment as well as to identifyG0 -positive cells population. Transcriptome profiling was provided to determine genes associated with dacarbazine resistance. We evaluated the activity of β-galactosidase in cells treated with dacarbazine by substrate hydrolysis. Cell adhesion strength was measured by centrifugal assay application with subsequent staining of adhesive cells with Ki-67 monoclonal antibodies. Ability of melanoma cells to metabolize dacarbazine was determined by expressional analysis of CYP1A1, CYP1A2, CYP2E1 followed by CYP1A1 protein level evaluation by the ELISA method.??Results: The present study determined that dacarbazine treatment of melanoma cells could induce an increase in the percentage of cells in G0 phase without alterations of β-galactosidase positive cells which corresponded to the fraction of the senescent cells. Transcriptomic profiling of cells under dacarbazine induction of G0 -positive cells percentage revealed that 'VEGFA-VEGFR2 signaling pathway' and 'Cell cycle' signaling were mostly enriched by dysregulated genes. 'Focal adhesion' signaling was also found to be triggered by dacarbazine. In melanoma cells treated with dacarbazine, an increase in G0 -positive cells among adherent cells was found.??Conclusions: Dacarbazine induces the alteration in a percentage of melanoma cells residing in G0 phase of a cell cycle. The altered adhesive phenotype of cancer cells under transition in the G0 phase may refer to a specific intercellular communication pattern of quiescent/senescent cancer cells.

Год издания: 2022

Авторы: Esimbekova Alexandra R. , Palkina Nadezhda V. , Zinchenko Ivan S. , Belenyuk Vasiliy D. , Savchenko Andrey A. , Sergeeva Ekaterina Yu , Ruksha Tatiana G.

Источник: Cancer Medicine

Номера страниц: 1-15

Держатель оригинала документа: Krasnoyarsk Science Centre of the Siberian Branch of Russian Academy of Science, Professor V.F. Voino-Yasenetsky Krasnoyarsk State Medical University

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